Differentiation of filoviruses by electron microscopy
Differentiation of filoviruses by electron microscopy
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DOI：
10.1016/0168-1702(95)00080-1
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发表时间：
1995-12-01
期刊：
影响因子：
5
通讯作者：
Jahrling, PB
中科院分区：
文献类型：
--
作者：
Geisbert, TW;Jahrling, PB
将培养的 MA-104、Vero 76、SW-13 和 DBS-FRhL-2 细胞单层感染马尔堡 (MEG)、埃博拉-苏丹 (EBO-S)、埃博拉-扎伊尔 (EBO-Z) 和埃博拉-雷斯顿 (EBO-R) 病毒（丝状病毒科、丝状病毒），并通过电子显微镜检查，以提供这些潜在人类的形态和形态发生的超微结构细节。病原体。在所有使用的细胞系统中都观察到每种丝状病毒的复制。丝状病毒颗粒似乎通过内吞作用进入宿主细胞。丝状病毒显示出相似的形态发生事件进展，从新生胞浆内病毒包涵体的出现到通过质膜出芽的成熟病毒体的形成，无论血清型或宿主细胞如何。然而，MBG 和其他丝状病毒之间存在超微结构差异。从培养液中回收的 MBG 病毒颗粒的平均单位长度均比 EBO-S、EBO-Z 或 EBO-R 颗粒短。对丝状病毒感染细胞的检查表明，中间 MBG 内含物在形态上与 EBO-S、EBO-Z 和 EBO-R 内含物不同。在 EBO-S、EBO-Z 和 EBO-R 之间未观察到病毒包涵体材料的结构差异。免疫电镜显示，丝状病毒基质蛋白（VP40）和核蛋白（NP）在EBO-Z包涵体中积累，并在病毒形态发生过程中密切相关。这些细节有助于通过电子显微镜对丝状病毒感染进行有效和明确的诊断。
Cultured monolayers of MA-104, Vero 76, SW-13, and DBS-FRhL-2 cells were infected with Marburg (MEG), Ebola-Sudan (EBO-S), Ebola-Zaire (EBO-Z), and Ebola-Reston (EBO-R) viruses (Filoviridae, Filovirus) and examined by electron microscopy to provide ultrastructural details of morphology and morphogenesis of these potential human pathogens. Replication of each filovirus was seen in all cell systems employed. Filoviral particles appeared to enter host cells by endocytosis. Filoviruses showed a similar progression of morphogenic events, from the appearance of nascent intracytoplasmic viral inclusions to formation of mature virions budded through plasma membranes, regardless of serotype or host cell. However, ultrastructural differences were demonstrated between MBG and other filoviruses. MBG virions recovered from culture fluids were uniformly shorter in mean unit length than EBO-S, EBO-Z, or EBO-R particles. Examination of filovirus-infected cells revealed that intermediate MBG inclusions were morphologically distinct from EBO-S, EBO-Z, and EBO-R inclusions. No structural difference of viral inclusion material was observed among EBO-S, EBO-Z, and EBO-R. Immunoelectron microscopy showed that the filoviral matrix protein (VP40) and nucleoprotein (NP) accumulated in EBO-Z inclusions, and were closely associated during viral morphogenesis. These details facilitate the efficient and definitive diagnosis of filoviral infections by electron microscopy.