Grafted poly-(ethylene glycol) on lipid surfaces inhibits protein adsorption and cell adhesion
Grafted poly-(ethylene glycol) on lipid surfaces inhibits protein adsorption and cell adhesion
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DOI：
10.1016/s0005-2736(97)00027-8
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发表时间：
1997-06-12
影响因子：
3.4
通讯作者：
Hui, SW
中科院分区：
文献类型：
--
作者：
Du, H;Chandaroy, P;Hui, SW
采用Langmuir-Blodgett法将双棕榈酰磷脂酰乙醇胺（DPPE）与不同摩尔百分比的二硬脂酰磷脂酰乙醇胺（DSPE）偶联聚乙二醇（PEG m.w. 750-5000）混合在dpe涂层的玻璃表面上沉积单层。在这些支持的脂质表面上增加接枝PEG的百分比，越来越多地抑制牛血清白蛋白（BSA）、层粘连蛋白和纤维连接蛋白的吸附。增加移植PEG的百分比也抑制红细胞、淋巴细胞和巨噬细胞对这些支持的脂质表面的粘附。用fitc标记蛋白的荧光检测蛋白在脂膜玻璃表面的吸附。细胞粘附主要通过显微计数测定。抑制红细胞粘附所需的PEG移植脂质浓度随着移植PEG分子量的增加而降低。抑制作用强烈依赖于低浓度PEG的接枝密度。但对接枝密度的依赖性较弱。对于DSPE-PEG5000，接枝密度依赖性的变化大约发生在接枝聚合物完全覆盖脂质表面时（0.7 mol%），并过渡到部分刷状构象。对于接枝分子量较低的聚乙二醇，转换变得不那么明显，可能是由于严格的蘑菇和刷状聚合物模型的失败。相对抑制效率取决于蛋白质或细胞。讨论了隐身脂质体功能的意义。(C) 1997爱思唯尔科学有限公司
Monolayers of dipalmitoyl-phosphatidylethanolamine (DPPE) mixing with various mole percentages of distearoyl-phosphatidylethanolamine (DSPE)-conjugated poly-(ethylene glycol) (PEG m.w. 750-5000) were deposited on DPPE-coated glass surfaces by the Langmuir-Blodgett method. Increasing percentages of grafted PEG in these supported lipid surfaces increasingly inhibit the adsorption of bovine serum albumin (BSA), laminin, and fibronectin. Increasing percentages of grafted PEG also inhibit the adhesion of erythrocytes, lymphocytes, and macrophages to these supported lipid surfaces. The adsorption of proteins on lipid coated glass surfaces were assayed by the fluorescence of FITC-labelled proteins. Cell adhesion was measured mainly by microscopic counting. The concentration of PEG-grafted lipids required for the inhibition of erythrocyte adhesion decreases with increasing molecular weight of the grafted PEG. The inhibitory effects are strongly dependent on the graft density of PEG at low concentrations? but weakly dependent on graft density at higher concentrations. For DSPE-PEG5000, the change of graft density dependency occurs approximately at the complete coverage of the lipid surface by the grafted polymer in the mushroom conformation (0.7 mol%), and the transition to partial brush conformation. The change-overs become less distinctive for grafted PEG of lower molecular weights, probably due to the failure of strictly mushroom and brush models of the polymer. The relative inhibitory efficiency is protein or cell dependent. The implication on the function of stealth liposomes is discussed. (C) 1997 Elsevier Science B.V.