SELECTIVE GROWTH IN SERUM-FREE HORMONE-SUPPLEMENTED MEDIUM OF TUMOR-CELLS OBTAINED BY BIOPSY FROM PATIENTS WITH SMALL CELL-CARCINOMA OF THE LUNG
SELECTIVE GROWTH IN SERUM-FREE HORMONE-SUPPLEMENTED MEDIUM OF TUMOR-CELLS OBTAINED BY BIOPSY FROM PATIENTS WITH SMALL CELL-CARCINOMA OF THE LUNG
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DOI：
10.1073/pnas.78.5.3185
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发表时间：
1981-01-01
期刊：
影响因子：
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通讯作者：
MINNA, JD
中科院分区：
文献类型：
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作者：
CARNEY, DN;BUNN, PA;MINNA, JD
直接从肺小细胞癌患者体内取15例肿瘤标本，检测其在含血清和无血清加氢化可的松、胰岛素、转铁蛋白、雌激素和硒(Hites)的培养液中的生长能力。15例中有14例(93%)在Hites培养液中有肿瘤细胞复制，在补充血清的培养液中有10例(67%)。在Hites培养液中生长的细胞通过标准细胞学标准、流式细胞仪DNA含量分析以及它们在琼脂糖凝胶和裸鼠肿瘤中形成集落的能力证实了肿瘤的起源。虽然肿瘤细胞在两种培养液中的形态非常相似，但无血清培养不支持非恶性基质细胞的生长，接种后7-10天可获得基本纯净的复制型肿瘤细胞培养。在小细胞癌细胞学阴性的20例标本和含有其他类型(包括其他类型的肺癌)的10例标本中，有9例未能生长，证明了Hites培养液的选择性。一种化学定义的介质，通过对组织培养适应的人类肿瘤株的研究而确定，可以支持直接从患者那里获得的肿瘤标本的选择性生长。这些选择性配方可能对不同的肿瘤类型具有特异性，因此可用于诊断、体外药敏试验和鉴定调节肿瘤生长的因素。所有这些都直接应用于患者的治疗。
Fifteen tumor-containing specimens were obtained directly from patients with small cell carcinoma of the lung and tested for their ability to grow in serum-supplemented medium and in serum-free medium supplemented with hydrocortisone, insulin, transferrin, estrogen and Se (HITES). The tumor cells replicated in 14 of 15 cases (93%) in the HITES medium and in 10 of 15 cases (67%) in the serum-supplemented medium. The neoplastic origin of the cells growing in the HITES medium was confirmed by standard cytologic criteria, by DNA content analysis using flow cytometry and by their ability to form colonies in agarose and tumors in athymic nude mice. While the tumor cells had very similar morphologies in both media, the serum-free medium did not support the growth of nonmalignant stromal cells and essentially pure cultures of replicating tumor cells were obtained 7-10 days after plating. The selectivity of the HITES medium was demonstrated by the failure of cells to grow in 20 specimens cytologically negative for small cell carcinoma and in 9 of 10 specimens containing other tumor types (including other types of lung cancer). A chemically defined medium, determined by work on tissue culture-adapted human tumor lines, can support the selective growth of tumor specimens obtained directly from patients. Such selective formulas are probably specific for different tumor types and thus could be used for diagnosis, drug sensitivity testing in vitro and identification of factors regulating tumor growth. All of these have direct application to patient treatment.