STRUCTURAL DIFFERENTIATION OF THE EPITHELIAL-CELLS OF THE TESTICULAR EXCURRENT DUCT SYSTEM OF RATS DURING POSTNATAL-DEVELOPMENT
STRUCTURAL DIFFERENTIATION OF THE EPITHELIAL-CELLS OF THE TESTICULAR EXCURRENT DUCT SYSTEM OF RATS DURING POSTNATAL-DEVELOPMENT
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DOI：
10.1002/ar.1092330205
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发表时间：
1992-06-01
影响因子：
--
通讯作者：
ROBAIRE, B
中科院分区：
文献类型：
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作者：
HERMO, L;BARIN, K;ROBAIRE, B
在出生后第21、39、49、56和90天，对大鼠附睾输出管和不同区域的各种上皮细胞进行了光镜和电镜检查，以确定雄激素和/或精子以及其他可能因素对这些细胞结构分化的作用。从这些观察中可以得出五个结论。首先，在第21天，所有区域的上皮细胞在结构上未分化。第二，直到第49天，传出导管的无纤毛细胞类似于成年动物的无纤毛细胞，这表明需要一个以上的因素，如雄激素，睾丸产品，和/或精子，其完整的结构分化。第三，附睾的主细胞在第39天开始结构分化，即，这些细胞含有精细的高尔基体、内质网池和许多200-400 nm电子透明的分泌囊泡，以及完整的内吞细胞器;尽管附睾腔中没有精子，但仍发生了这种情况。这些附睾细胞的分化可能受到雄激素的影响，已知此时雄激素水平较高，但也可能是由于支持细胞直接分泌到输出管中的特异性分泌物。第四，附睾尾的透明细胞在第39天完全分化。附睾尾管腔中存在变性生殖细胞和各种细胞碎片，以及高雄激素水平，可能是导致该区域细胞分化的因素。最后，透明细胞的附睾体和尾部只有成为完全分化的49天，在精子出现在管腔的时候，尽管在39天的雄激素水平高，这一观察表明，精子在管腔中的存在可能是一个必要的因素，在导致其分化。总之，这些结果表明，不同因素的组合是必要的附睾不同区域的各种上皮细胞类型的结构分化。
The light and electron microscopic appearance of the various epithelial cells lining the efferent ducts and different regions of the epididymis were examined in rats on postnatal days 21, 39, 49, 56, and 90 to determine the role of androgens and/or spermatozoa, as well as other possible factors, on the structural differentiation of these cells. Five conclusions may be drawn from the observations made. First, on day 21 epithelial cells of all regions are structurally undifferentiated. Second, it was not until day 49 that nonciliated cells of the efferent ducts resembled those of adult animals, suggesting that more than one factor, such as androgens, testicular products, and/or spermatozoa, is needed for their full structural differentiation. Third, principal cells of the epididymis become structurally differentiated by day 39, i.e., these cells contained an elaborate Golgi apparatus, endoplasmic reticulum cisternae, and numerous 200-400 nm electron lucent secretory vesicles, as well as a full complement of endocytic organelles; this occurred in spite of the absence of spermatozoa in the epididymal lumen. The differentiation of these epididymal cells may be under the influence of androgens, which are known to be high at this time, but may also be due to specific secretions from Sertoli cells secreted directly into the efferent ducts. Fourth, clear cells of the cauda epididymidis are fully differentiated by day 39. The presence of degenerating germ cells in the lumen of the cauda epididymidis and various cellular debris, as well as high androgen levels, may be factors causing the differentiation of the cells of this region. Finally, clear cells of the corpus and cauda epididymidis only become fully differentiated by day 49, at a time when spermatozoa appear in the lumen, despite high levels of androgens at day 39; this observation indicates that the presence of spermatozoa in the lumen may be a necessary factor in causing their differentiation. Overall, these results suggest that a combination of different factors are necessary for the structural differentiation of the various epithelial cell types of the different regions of the epididymis.