An evaluation of the effects of Lactobacillus ingluviei on body weight, the intestinal microbiome and metabolism in mice
An evaluation of the effects of Lactobacillus ingluviei on body weight, the intestinal microbiome and metabolism in mice
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DOI：
10.1016/j.micpath.2011.10.004
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发表时间：
2012-01-01
影响因子：
3.8
通讯作者：
Raoult, Didier
中科院分区：
文献类型：
--
作者：
Angelakis, Emmanouil;Bastelica, Delphine;Raoult, Didier
背景资料：食物可以改变肠道植物群，并且已经显示出在鸡和鸭中但在哺乳动物中不引起增重的乳杆菌（Lactobacillus ingluviei）。或者PBS。收集粪便样品并使用qPCR进行测试，以检测和定量乳杆菌属，拟杆菌属和厚壁菌属（Firmicutes spp.）通过微阵列和qPCR检测肝脏和脂肪组织中的基因表达。结果：小鼠接种4 × 10（10）L. ingluviei的体重增加和肝脏重量显著增加，乳杆菌属的显著增加。和厚壁菌门的DNA拷贝数采用RT-PCR方法检测了30例正常人肝组织中脂肪酰基合成酶（Fas）、固醇调节元件结合因子1（Srebp 1c）、肿瘤坏死因子α（Tnf）、细胞色素P450 2 E1（Cyp 2 e1）、3-磷酸肌醇依赖性蛋白激酶1（Pdpk 1）、酰基辅酶A脱氢酶11（Acad 11）、实验组动物肝组织中ATP结合盒亚家族成员G（ABCG 2）和DEAD盒多肽25（Ddx 25）明显升高。在性腺脂肪组织中，实验组动物的瘦素、过氧化物酶体增殖物激活受体γ（Pparg）和Srebp 1c的表达水平显着较高，而脂联素的表达显着较低。结论：接种L.在小鼠中的ingluviei导致肠道植物群的改变、增加的体重增加和肝脏增大、加速的代谢和增加的炎症。(C)2011爱思唯尔有限公司保留所有权利。
Background: Food can modify the intestinal flora, and Lactobacillus ingluviei has been shown to cause weight gain in chicks and ducks but not in mammals.Methodology: Female BALB/c mice were divided into a control and two experimental groups and were inoculated either once or twice with L. ingluviei or with PBS. Faecal samples were collected and tested using qPCR in order to detect and quantify Lactobacillus spp., Bacteroidetes spp. and Firmicutes spp. Gene expression was examined in liver and adipose tissue by microarray and qPCR. Metabolic indicators in the plasma were also measured.Results: Mice that were inoculated with 4 x 10(10) L. ingluviei presented a significant increase in weight gain and liver weight and significant increases in Lactobacillus spp. and Firmicutes DNA copy numbers in their faeces. The mRNA levels of fatty acyl synthase (Fas), sterol regulatory element binding factor 1 (Srebp1c), tumour necrosis factor alpha (Tnf), cytochrome P450 2E1 (Cyp2e1), 3-phosphoinositide-dependent protein kinase-1 (Pdpk1), acyl-Coenzyme A dehydrogenase 11 (Acad11), ATP-binding cassette sub family member G (ABCG2) and DEAD box polypeptide 25 (Ddx25) were significantly elevated in the liver tissues of animals in the experimental group. In gonadal adipose tissue, the expression levels of leptin, peroxisome proliferator-activated receptor gamma (Pparg) and Srebp1c were significantly higher in animals from the experimental group, whereas the expression of adiponectin was significantly lower in these animals.Conclusions: The inoculation of L. ingluviei in mice resulted in alterations in the intestinal flora, increased weight gain and liver enlargement, accelerated metabolism and increased inflammation. (C) 2011 Elsevier Ltd. All rights reserved.