DNA acts as a nucleation site for transient cavitation in the ultrasonic nebulizer
DNA acts as a nucleation site for transient cavitation in the ultrasonic nebulizer
复制标题
DOI：
10.1002/jps.20511
复制
发表时间：
2006-03-01
影响因子：
3.8
通讯作者：
Lengsfeld, CS
中科院分区：
文献类型：
--
作者：
Lentz, YK;Anchordoquy, TJ;Lengsfeld, CS
已经提出了基于超声技术的几种新技术作为增强遗传疗法递送的方法。利用超声雾化和一种成熟的方法来定量监测瞬态空化，本研究探讨了影响DNA降解的程度和因素。从我们的研究结果表明，DNA的存在下，大大提高了空化，和瞬态空化事件的数量也增加与流体动力学直径和DNA分子在溶液中的数量。更重要的是，从质粒DNA溶液中去除饱和气体导致瞬时空化事件减少，但降解速率没有减少，这表明导致降解的空化事件局部发生在DNA分子上。最后，复合质粒DNA与阳离子聚合物聚乙烯亚胺保护的天然结构，通过减少分子的潜力，作为一个非均质成核位点的瞬态空化。(c)2006 Wiley-Liss，Inc.和美国药剂师协会
Several new technologies based upon ultrasound technology have been proposed as a method to enhance the delivery of genetic therapeutics. Using ultrasonic nebulization and a well-established method to quantitatively monitor transient cavitation, this study investigates the extent and factors influencing the degradation of DNA. Results from our studies show that the presence of DNA greatly enhances cavitation, and that the number of transient cavitation events also increases with the hydrodynamic diameter and number of DNA molecules in solution. More importantly, removing saturated gases from the plasmid DNA solutions resulted in a decrease in transient cavitation events but not degradation rate, suggesting that the cavitation event responsible for degradation occurs locally at the DNA molecule. Finally, complexing plasmid DNA with the cationic polymer polyethylenimine protected the native structure by reducing the molecule's potential to act as a heterogeneous nucleation site for transient cavitation. (c) 2006 Wiley-Liss, Inc. and the American Pharmacists Association.