PROPIDIUM IODIDE AS AN INDICATOR OF GIARDIA CYST VIABILITY
PROPIDIUM IODIDE AS AN INDICATOR OF GIARDIA CYST VIABILITY
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DOI：
10.1128/aem.57.11.3243-3247.1991
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发表时间：
1991-11-01
影响因子：
4.4
通讯作者：
JAKUBOWSKI, W
中科院分区：
文献类型：
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作者：
SAUCH, JF;FLANIGAN, D;JAKUBOWSKI, W
研究了碘化丙啶的使用，碘化丙啶的摄取指示细胞死亡或损伤，以评估热灭活和化学灭活的鼠贾第鞭毛虫包囊的活力。 这是通过比较碘化丙啶染色与脱囊来完成的。 我们首先确定，碘化丙啶可用于免疫荧光检测程序，通过显示蓝氏贾第鞭毛虫包囊染色这种染料的百分比之前和之后进行荧光检测方法是相似的。 G.然后将鼠孢囊暴露于热（56 ℃）、每升0.5至4mg氯（pH 7.0，5 ℃）、每升0.1至10mg季铵化合物或每升2mg预形成和形成的一氯胺（pH 7.2，18至20 ℃）。 碘化丙啶染色的囊肿百分比和无脱囊率之间有很好的正相关性。暴露于热或暴露于季铵化合物的小鼠包囊。 然而，无脱囊和碘化丙啶染色之间没有显着的相关性被发现暴露于氯或一氯胺的囊肿。 这些结果表明碘化丙啶染色法不能满足G.这两种常用的饮用水消毒剂。
The use of propidium iodide, whose uptake indicates cell death or damage, was investigated to assess the viability of heat-inactivated and chemically inactivated Giardia muris cysts. This was done by comparing propidium iodide staining with excystation. We first determined that propidium iodide could be used with an immunofluorescence detection procedure by showing that the percentages of Giardia lamblia cysts stained with this dye before and after subjecting them to a fluorescence detection method were similar. G. muris cysts were then exposed to heat (56-degrees-C), 0.5 to 4 mg of chlorine per liter (pH 7.0, 5-degrees-C), 0.1 to 10 mg of a quaternary ammonium compound per liter, or 2 mg of preformed and forming monochloramine per liter (pH 7.2, 18 to 20-degrees-C). A good positive correlation between percent propidium iodide-stained cysts and lack of excystation was demonstrated for G. muris cysts exposed either to heat or to the quaternary ammonium compound. However, no significant correlation between absence of excystation and propidium iodide staining was found for cysts exposed to chlorine or monochloramines. These results demonstrate that the propidium iodide staining procedure is not satisfactory for determining the viability of G. muris cysts exposed to these two commonly used drinking water disinfectants.