ACE1 REGULATES EXPRESSION OF THE SACCHAROMYCES-CEREVISIAE METALLOTHIONEIN GENE
ACE1 REGULATES EXPRESSION OF THE SACCHAROMYCES-CEREVISIAE METALLOTHIONEIN GENE
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DOI：
10.1128/mcb.8.7.2745
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发表时间：
1988-07-01
影响因子：
5.3
通讯作者：
THIELE, DJ
中科院分区：
文献类型：
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作者：
THIELE, DJ
酿酒酵母对铜的抗性在很大程度上是由编码低分子量、富含半胱氨酸的金属结合蛋白的CUP1基因座介导的。CUP1基因的表达是在转录诱导水平上调节的，以响应环境中高水平的铜。本文报道了一株酵母突变株ACE1-1的分离，该突变株在暴露于外源铜时存在激活CUP1表达的缺陷。ACE1-1突变是隐性的，存在于编码反式作用CUP1调节因子的遗传元件中。通过体内互补获得了野生型ACE1基因，并恢复了铜诱导的CUP1表达和铜对铜敏感的ACE1-1突变体的抗性。连锁分析和基因缺失实验证明，该基因代表了真正的ACE1基因座。ACE1位于第七染色体的左臂，9个着丝粒器官位于5号染色体的远端。ACE1基因似乎在铜浓度升高时激活CUP1的表达中起到直接或间接的积极作用。
Copper resistance in Saccharomyces cerevisiae is mediated, in large part, by the CUP1 locus, which encodes a low-molecular-weight, cysteine-rich metal-binding protein. Expression of the CUP1 gene is regulated at the level of transcriptional induction in response to high environmental copper levels. This report describes the isolation of a yeast mutant, ace1-1, which is defective in the activation of CUP1 expression upon exposure to exogenous copper. The ace1-1 mutation is recessive and lies in a genetic element that encodes a trans-acting CUP1 regulatory factor. The wild-type ACE1 gene was isolated by in vivo complementation and restores copper inducibility of CUP1 expression and copper resistance to the otherwise copper-sensitive ace1-1 mutant. Linkage analysis and gene deletion experiments verified that this gene represents the authentic ACE1 locus. ACE1 maps to the left arm of chromosome VII, 9 centimorgans centromere distal to lys5. The ACE1 gene appears to play a direct or indirect positive role in activation of CUP1 expression in response to elevated copper concentrations.