Cross-resistance patterns to acetyl coenzyme A carboxylase (ACCase) inhibitors associated with different ACCase mutations in Beckmannia syzigachne
Cross-resistance patterns to acetyl coenzyme A carboxylase (ACCase) inhibitors associated with different ACCase mutations in Beckmannia syzigachne
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DOI：
10.1111/wre.12170
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发表时间：
2015-12-01
期刊：
影响因子：
1.7
通讯作者：
Dong, L. Y.
中科院分区：
文献类型：
--
作者：
Pan, L.;Li, J.;Dong, L. Y.
Beckmannia syzigachne（美国腐草）是在中国发现的一种竞争性禾本科杂草。精恶唑禾草灵在中国被广泛用于控制该物种。据报道，丁香小蠹对精恶唑禾草灵的抗性是由编码除草剂靶标乙酰辅酶A羧化酶 (ACCase) 的基因中的异亮氨酸 (Ile)-1781-亮氨酸 (Leu) 取代赋予的。本研究通过派生切割扩增多态性序列（dCAPS）方法在抗精恶唑禾草灵群体中检测到三个突变：JCWL-R群体中的Ile-1781-Leu、JCJT-R中的Ile-2041-Asn和JYJD-R中的Gly-2096-Ala。数据表明它们在 ACCase 基因座上具有遗传同质性（纯合突变体）。细胞色素 P450 抑制剂的使用显示出轻微降低了精恶唑禾草灵耐药群体的 GR(50) 值，由此我们推断，靶点耐药性 (TSR) 和非靶点耐药性 (NTSR) 的组合参与了精恶唑禾草灵耐药性。我们描述了丁香小蠹对 ACCase 抑制剂的交叉耐药模式。 JCWL-R群体中的植物对所有测试的APP（芳氧基苯氧基丙酸酯）、稀禾定和唑啉草酯均具有高度抗性，对烯草酮具有中度抗性。 JCJT-R群体中的植物对氟啶草酯、炔草酯、氰氟草酯、恶唑草酯和唑啉草酯具有高度抗性；对右旋氟草灵、精喹禾灵和稀禾定中度耐药；对烯草酮敏感。 JYJD-R群体中的植物对炔草酯、恶唑草酯和唑啉草酯具有高度抗性；对氟草灵、氰氟草酯、精喹禾灵、氟啶草灵和稀禾定中度耐药；对烯草酮敏感。如果田间的丁香小蠹种群中存在对 ACCase 抑制剂的抗性，那么我们的结果表明应使用烯草酮。虽然我们证明了丁香小蠹中的三个突变导致的 TSR 交叉抗性模式，但我们还证明了 NTSR 在抗性中发挥着作用，这将使杂草管理变得复杂。
Beckmannia syzigachne (American sloughgrass) is a competitive grass weed found in China. Fenoxaprop-P-ethyl is widely used for control of this species in China. Resistance to fenoxaprop-P-ethyl in B. syzigachne has been reported to be conferred by an isoleucine(Ile)-1781-leucine(Leu) substitution in the gene encoding the herbicide target, acetyl-CoA carboxylase (ACCase). In this study, three mutations were detected by derived cleaved amplified polymorphic sequence (dCAPS) method in fenoxaprop-P-ethyl-resistant B. syzigachne populations: Ile-1781-Leu in population JCWL-R, Ile-2041-Asn in JCJT-R and Gly-2096-Ala in JYJD-R. The data indicated they were genetically homogeneous (homozygous mutant) at the ACCase locus. The use of cytochrome P450 inhibitors was shown to slightly reduce the GR(50) value of fenoxaprop-P-ethyl-resistant populations, from which we inferred a combination of target-site resistance (TSR) and non-target-site resistance (NTSR) was involved in fenoxaprop-P-ethyl-resistance. We characterised the cross-resistance patterns to ACCase inhibitors in B. syzigachne. The plants in the JCWL-R population were highly resistant to all tested APPs (aryloxyphen-oxypropionates), sethoxydim and pinoxaden, and moderately resistant to clethodim. The plants in the JCJT-R population were highly resistant to fluazifop-P-butyl, clodinafop-propargyl, cyhalo-fop-butyl, metamifop and pinoxaden; moderately resistant to haloxyfop-R-methyl, quizalofop-P-ethyl and sethoxydim; and sensitive to clethodim. The plants in the JYJD-R population were highly resistant to clodinafop-propargyl, metamifop and pinoxaden; moderately resistant to haloxyfop-R-methyl, cyhalofop-butyl, quizalofop-P-ethyl, fluazifop-P-butyl and sethoxydim; and sensitive to clethodim. If resistance to ACCase inhibitors is present in B. syzigachne populations in the field, then our results indicate that clethodim should be used. While we demonstrated the cross-resistance patterns of TSR resulting from three mutations in B. syzigachne, we also demonstrated that NTSR plays a role in resistance, which will complicate weed management.