Hypoxia exposure and B-type natriuretic peptide release from Langendorff heart of rats
Hypoxia exposure and B-type natriuretic peptide release from Langendorff heart of rats
复制标题
DOI：
10.1111/apha.12767
复制
发表时间：
2017-05-01
影响因子：
6.3
通讯作者：
Nikinmaa, M.
中科院分区：
文献类型：
--
作者：
Anttila, K.;Streng, T.;Nikinmaa, M.
Aim我们研究了是否可用的氧气没有诱导的机械拉伸调节释放的生物活性的B型利钠肽（BNP）从Langendorff heart.MethodsRat心脏分离和灌注生理Krebs-Henseleit解决方案在一个恒定的静水压力在Langendorff设置。灌注液基础O2浓度（24.4 ± 0.04mgL（-1））在20 min内逐渐降至3.0 ± 0.01mgL（-1）（n=7）。测定冠脉血流中BNP和O-2水平。对照组（n=5），氧浓度维持在26.6 +/- 0.3mgL（-1）。
AimWe studied whether available oxygen without induced mechanical stretch regulates the release of the biologically active B-type natriuretic peptide (BNP) from Langendorff heart.MethodsRat hearts were isolated and perfused with a physiological Krebs-Henseleit solution at a constant hydrostatic pressure in Langendorff set-up. The basal O-2 level of perfusate (24.40.04mgL(-1)) was gradually lowered to 3.00.01mgL(-1) over 20min using N-2 gas (n=7). BNP and O-2 level were measured from coronary flow. During control perfusions (n=5), the O-2 concentration was kept at 26.6 +/- 0.3mgL(-1).ResultsA low oxygen concentration in the perfusate was associated with a significant increase in BNP release (F=40.4, P