Hyaluronan Modulates Proliferation and Migration of Rabbit Fibroblasts Derived From Flexor Tendon Epitenon and Endotenon
Hyaluronan Modulates Proliferation and Migration of Rabbit Fibroblasts Derived From Flexor Tendon Epitenon and Endotenon
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DOI：
10.1016/j.jhsa.2010.02.010
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发表时间：
2010-05-01
影响因子：
1.9
通讯作者：
Nagata, Kensei
中科院分区：
文献类型：
--
作者：
Yagi, Masaharu;Sato, Naoto;Nagata, Kensei
目的越来越多的证据支持使用透明质酸(HA)治疗肌腱损伤，尽管其愈合效果的机制尚不清楚。因此，我们研究了透明质酸对兔屈肌腱外膜和肌腱成纤维细胞增殖和迁移的影响。方法取兔滑膜内肌腱(n=5)，培养肌腱成纤维细胞。用流式细胞仪检测肌腱成纤维细胞CD44的表达。在单层培养的肌腱成纤维细胞中加入不同浓度的HA(0.1~5.0 mg/mL)。通过记录细胞数量的变化来评估细胞的增殖情况，并通过伤口愈合实验来测量细胞的迁移。不同浓度的透明质酸可明显抑制细胞增殖，促进细胞迁移，且呈剂量依赖性。结论透明质酸对兔屈肌腱外膜和肌腱内膜成纤维细胞的增殖和迁移有调节作用。J Hand Surg 2010；35A：791-796。版权所有(C)2010年，由美国手外科学会提供。保留所有权利。)
Purpose There is a growing body of evidence supporting the use of hyaluronan (HA) for treatment of injured tendons, although the mechanism of the healing effect has not yet been clarified. We therefore investigated the effects of HA on the proliferation and migration of tendon fibroblasts derived from rabbit flexor tendon epitenon and endotenon.Methods From explanted rabbit intrasynovial flexor tendons (n = 5), we cultured tendon fibroblasts derived from the epitenon and endotenon. CD44 expression on the tendon fibroblasts was detected by flow cytometric analysis. Various concentrations of HA (0.1-5.0 mg/mL) were added to monolayer-cultured tendon fibroblasts. We evaluated cell proliferation by recording changes in cell number, and measured cell migration by wound-healing assay.Results Flow cytometric analysis detected CD44 expression on the tendon fibroblasts. Treatment with HA at various concentrations notably and dose dependently inhibited cell proliferation and promoted cell migration.Conclusions Hyaluronan modulates the proliferation and migration of rabbit fibroblasts derived from the flexor tendon epitenon and endotenon. (J Hand Surg 2010;35A:791-796. Copyright (C) 2010 by the American Society for Surgery of the Hand. All rights reserved.)