NUCLEAR FACTOR-I IS SPECIFICALLY TARGETED TO DISCRETE SUBNUCLEAR SITES IN ADENOVIRUS TYPE-2-INFECTED CELLS
NUCLEAR FACTOR-I IS SPECIFICALLY TARGETED TO DISCRETE SUBNUCLEAR SITES IN ADENOVIRUS TYPE-2-INFECTED CELLS
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DOI：
10.1128/jvi.66.5.3140-3150.1992
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发表时间：
1992-05-01
影响因子：
5.4
通讯作者：
HAY, RT
中科院分区：
文献类型：
--
作者：
BOSHER, J;DAWSON, A;HAY, RT
在真核细胞周期的S期和病毒感染的细胞中，DNA复制发生在细胞核中的离散位点，尽管尚不清楚参与复制过程的蛋白质如何定向到这些位点。 核因子I是一种细胞序列特异性DNA结合蛋白，被2型腺病毒利用，以促进核蛋白复合物在病毒DNA复制起点的组装。 免疫荧光实验表明，在未感染的细胞中，核因子I均匀分布在整个细胞核中。 然而，在细胞被2型腺病毒感染后，核因子I的分布发生了显着改变，与病毒DNA结合蛋白共定位在有限数量的亚核位点中，溴脱氧尿苷脉冲标记实验已将其鉴定为病毒DNA复制的位点。 4型腺病毒不需要核因子I进行病毒DNA复制，其实验表明，尽管4型腺病毒DNA结合蛋白定位于离散的核位点，但这不会导致核因子I的重新分布。 因此，核因子I定位于离散的亚核位点可能代表了反映2型腺病毒DNA复制中对核因子I的需要的特异性靶向事件。
During the S phase of the eukaryotic cell cycle and in virus-infected cells, DNA replication takes place at discrete sites in the nucleus, although it is not clear how the proteins involved in the replicative process are directed to these sites. Nuclear factor I is a cellular, sequence-specific DNA-binding protein utilized by adenovirus type 2 to facilitate the assembly of a nucleoprotein complex at the viral origin of DNA replication. Immunofluorescence experiments reveal that in uninfected cells, nuclear factor I is distributed evenly throughout the nucleus. However, after a cell is infected with adenovirus type 2, the distribution of nuclear factor I is dramatically altered, being colocalized with the viral DNA-binding protein in a limited number of subnuclear sites which bromodeoxyuridine pulse-labeling experiments have identified as sites of viral DNA replication. Experiments with adenovirus type 4, which does not require nuclear factor I for viral DNA replication, indicate that although the adenovirus type 4 DNA-binding protein is localized to discrete nuclear sites, this does not result in the redistribution of nuclear factor I. Localization of nuclear factor I to discrete subnuclear sites is therefore likely to represent a specific targeting event that reflects the requirement for nuclear factor I in adenovirus type 2 DNA replication.