Insight into metabolic pathways of the potential biofuel producer, Paenibacillus polymyxa ICGEB2008.
Insight into metabolic pathways of the potential biofuel producer, Paenibacillus polymyxa ICGEB2008.
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DOI：
10.1186/s13068-015-0338-4
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发表时间：
2015
影响因子：
6.3
通讯作者：
Yazdani SS
中科院分区：
文献类型：
--
作者：
Adlakha N;Pfau T;Ebenhöh O;Yazdani SS
多粘类芽孢杆菌（Paenibacillus polymyxa）是一种兼性厌氧微生物，以生产水解酶和各种重要的生物燃料分子而闻名。尽管其广泛的工业用途和其基因组序列的可用性，但对类芽孢杆菌系统中的代谢途径知之甚少。在这里，我们报告的昆虫肠道共生体，多粘类芽孢杆菌ICGEB 2008的代谢见解，并揭示在生产2，3-丁二醇和乙醇中发挥重要作用的途径。我们开发了多粘原杆菌ICGEB 2008的代谢网络模型，其中包含133种代谢物和158种反应。通量平衡分析被用来研究氧化还原平衡在ICGEB 2008中的重要性。这导致检测到Bioblasts分流，这是一种以前未在类芽孢杆菌属中描述的途径，它可以将ATP的产生与还原当量的产生解偶联。使用实验和建模相结合的方法，我们进一步研究了参与2，3-丁二醇和乙醇生产的途径，并证明了生物体的氢气生产。我们可以进一步证明氮源对于类芽孢杆菌属的代谢物产生是关键的，并且正确地量化对ICGEB 2008的副产物代谢物谱的影响。模拟和实验表明，代谢通量从乙醇转向乙酸生产时，利用氧化氮源。我们已经建立了一个预测模型的中央碳代谢的多粘类囊原虫ICGEB 2008，并可以显示存在的Bioprosthunt分流，并解释其在ICGEB 2008的作用。我们进行了深入的研究，以了解乙醇、2，3-丁二醇和氢生产所涉及的代谢途径，这可用作进一步代谢工程工作的基础，以提高该多粘假单胞菌菌株的生物燃料生产效率。本文的在线版本（doi：10.1186/s13068-015-0338-4）包含补充材料，可供授权用户使用。
Paenibacillus polymyxa is a facultative anaerobe known for production of hydrolytic enzymes and various important biofuel molecules. Despite its wide industrial use and the availability of its genome sequence, very little is known about metabolic pathways operative in the Paenibacillus system. Here, we report metabolic insights of an insect gut symbiont, Paenibacillus polymyxa ICGEB2008, and reveal pathways playing an important role in the production of 2,3-butanediol and ethanol. We developed a metabolic network model of P. polymyxa ICGEB2008 with 133 metabolites and 158 reactions. Flux balance analysis was employed to investigate the importance of redox balance in ICGEB2008. This led to the detection of the Bifid shunt, a pathway previously not described in Paenibacillus, which can uncouple the production of ATP from the generation of reducing equivalents. Using a combined experimental and modeling approach, we further studied pathways involved in 2,3-butanediol and ethanol production and also demonstrated the production of hydrogen by the organism. We could further show that the nitrogen source is critical for metabolite production by Paenibacillus, and correctly quantify the influence on the by-product metabolite profile of ICGEB2008. Both simulations and experiments showed that metabolic flux is diverted from ethanol to acetate production when an oxidized nitrogen source is utilized. We have created a predictive model of the central carbon metabolism of P. polymyxa ICGEB2008 and could show the presence of the Bifid shunt and explain its role in ICGEB2008. An in-depth study has been performed to understand the metabolic pathways involved in ethanol, 2,3-butanediol and hydrogen production, which can be utilized as a basis for further metabolic engineering efforts to improve the efficiency of biofuel production by this P. polymyxa strain. The online version of this article (doi:10.1186/s13068-015-0338-4) contains supplementary material, which is available to authorized users.