Specific elements of the glyoxylate pathway play a significant role in the functional transition of the soybean cotyledon during seedling development.
Specific elements of the glyoxylate pathway play a significant role in the functional transition of the soybean cotyledon during seedling development.
复制标题
乙氧基途径的特定元素在幼苗发育过程中大豆子叶的功能过渡中起着重要作用。
DOI：
10.1186/1471-2164-8-468
复制
发表时间：
2007-12-19
期刊：
影响因子：
4.4
通讯作者：
Vodkin, Lila O
中科院分区：
文献类型：
--
作者：
Gonzalez, Delkin O;Vodkin, Lila O
大豆（Glycine max）子叶是一种特化的组织，其主要功能是作为营养储备，在整个幼苗发育过程中供应幼苗的需要。在这个过程中，子叶经历了一个功能转变，主要是光合组织。为了在遗传水平上鉴定参与子叶从储存到光合活性的自然过渡的特定活性元件，我们使用含有19，200个探针（70-mer长）的新大豆寡核苷酸芯片研究了不同时间点的转录本丰度谱。在标准化和统计分析后，我们确定了3，594个基因在研究定义的至少一个时间点中相对于吸胀种子呈现统计学显著改变的表达。这些数据的详细分析确定了个别的，具体的乙醛酸途径的元素，在子叶从营养物质储存到光合作用的功能转变过程中发挥了重要作用。在这一系列事件中，乙醛酸酶体和过氧化物酶体之间的动力学是明显的。我们还确定了其他几个基因，其产物可以协调地参与整个功能转换和相关的控制和调节机制，我们描述了多个未知的遗传元件，通过关联有可能对这一生物过程做出重大贡献。我们证明了大豆子叶在幼苗发育过程中的整体转录谱是非常活跃的，高度调节的和动态的。我们定义了单个基因家族成员、酶同工型和蛋白质亚基的表达谱，并根据它们参与大豆幼苗发育和子叶功能转变相关的不同功能活动，特别是与乙醛酸循环相关的功能活动，对它们进行了分类。我们的数据表明，在大豆子叶一个非常复杂的和同步的控制和调节系统的几个代谢途径是必不可少的，在这个发育过程中进行必要的功能。
The soybean (Glycine max) cotyledon is a specialized tissue whose main function is to serve as a nutrient reserve that supplies the needs of the young plant throughout seedling development. During this process the cotyledons experience a functional transition to a mainly photosynthetic tissue. To identify at the genetic level the specific active elements that participate in the natural transition of the cotyledon from storage to photosynthetic activity, we studied the transcript abundance profile at different time points using a new soybean oligonucleotide chip containing 19,200 probes (70-mer long). After normalization and statistical analysis we determined that 3,594 genes presented a statistically significant altered expression in relation to the imbibed seed in at least one of the time points defined for the study. Detailed analysis of this data identified individual, specific elements of the glyoxylate pathway that play a fundamental role during the functional transition of the cotyledon from nutrient storage to photosynthesis. The dynamics between glyoxysomes and peroxisomes is evident during these series of events. We also identified several other genes whose products could participate co-ordinately throughout the functional transition and the associated mechanisms of control and regulation and we described multiple unknown genetic elements that by association have the potential to make a major contribution to this biological process. We demonstrate that the global transcript profile of the soybean cotyledon during seedling development is extremely active, highly regulated and dynamic. We defined the expression profiles of individual gene family members, enzymatic isoforms and protein subunits and classified them accordingly to their involvement in different functional activities relevant to seedling development and the cotyledonary functional transition in soybean, especially the ones associated with the glyoxylate cycle. Our data suggests that in the soybean cotyledon a very complex and synchronized system of control and regulation of several metabolic pathways is essential to carry out the necessary functions during this developmental process.