Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators.
Functional optoacoustic neuro-tomography for scalable whole-brain monitoring of calcium indicators.
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DOI：
10.1038/lsa.2016.201
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发表时间：
2016-12
期刊：
影响因子：
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通讯作者：
Razansky D
中科院分区：
文献类型：
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作者：
Deán-Ben XL;Sela G;Lauri A;Kneipp M;Ntziachristos V;Westmeyer GG;Shoham S;Razansky D
非侵入性地观察分布在整个大脑中的大型神经群体的时空活动是神经科学的长期目标。我们开发了一个体积多光谱光声断层成像平台，用于成像散射大脑深处的神经激活。它可以在50至1000 mm 3的可扩展视场内每秒记录100个体积帧，相应的空间分辨率为35-200 μm。在固定和自由游泳的幼虫和成年斑马鱼大脑中表达遗传编码的钙指示剂GCaMP 5G的实验首次证明了使用光声直接跟踪神经动力学的基本能力，同时克服了散射大脑中光学成像的长期渗透障碍。因此，新开发的平台为快速钙动力学的功能性全脑观察提供了前所未有的能力;结合光声学在解决血管血流动力学方面的成熟能力，它可以在健康和疾病的神经活动和神经血管耦合研究中开辟新的前景。
Non-invasive observation of spatiotemporal activity of large neural populations distributed over entire brains is a longstanding goal of neuroscience. We developed a volumetric multispectral optoacoustic tomography platform for imaging neural activation deep in scattering brains. It can record 100 volumetric frames per second across scalable fields of view ranging between 50 and 1000 mm3 with respective spatial resolution of 35–200 μm. Experiments performed in immobilized and freely swimming larvae and in adult zebrafish brains expressing the genetically encoded calcium indicator GCaMP5G demonstrate, for the first time, the fundamental ability to directly track neural dynamics using optoacoustics while overcoming the longstanding penetration barrier of optical imaging in scattering brains. The newly developed platform thus offers unprecedented capabilities for functional whole-brain observations of fast calcium dynamics; in combination with optoacoustics' well-established capacity for resolving vascular hemodynamics, it could open new vistas in the study of neural activity and neurovascular coupling in health and disease.