alpha-Actinin localization in the junctional complex of intestinal epithelial cells.
alpha-Actinin localization in the junctional complex of intestinal epithelial cells.
复制标题
α-肌动蛋白在肠上皮细胞连接络合物中的定位。
DOI：
10.1083/jcb.80.1.203
复制
发表时间：
1979-01
期刊：
影响因子：
--
通讯作者：
Pardo JV
中科院分区：
文献类型：
--
作者：
Craig SW;Pardo JV
We have used antibody to chicken gizzard alpha-actinin to identify and localize this molecule in chicken intestinal epithelium. The antibody binds only to alpha-actinin when tested against a crude extract of chicken gizzard. Extracts of purified epithelial cells contain a molecule which has a subunit molecular weight of 100,000 on sodium dodecyl sulphate gels and which is able to inhibit the interaction of alpha-actinin antibody and 125I-labeled chicken gizzard alpha-actinin. By indirect immunofluorescence, alpha-actinin is localized in the apical portion of chicken intestinal epithelial cells. Ethanol-fixed cryostat sections of intestine taken through the apical portion of the epithelial cells and in a plane perpendicular to the long axis of the cells show that alpha-actinin is organized in a polygonal pattern which corresponds to the outlines of the polygonally packed epithelial cells. We interpret the data as indicating that alpha-actinin is a component of the tight junction (zonula occludens) and/or the belt desmosome (zonula adherens), both of which are membrane structures known to encircle the cell and to be confined to its apical portion.
