Human tyrosine tRNA is also internally cleavable by E-coli ribonuclease P RNA ribozyme in vitro
Human tyrosine tRNA is also internally cleavable by E-coli ribonuclease P RNA ribozyme in vitro
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DOI：
10.1271/bbb.65.2798
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发表时间：
2001-12-01
影响因子：
1.6
通讯作者：
Kikuchi, Y
中科院分区：
文献类型：
--
作者：
Ando, T;Tanaka, T;Kikuchi, Y
转运RNA是生物系统中必不可少的分子，每个转运RNA分子通常具有三叶草结构。以前，我们通过实验证明，一些果蝇tRNA（tRNA（Ala），tRNA（His）和tRNA（i）（Met））分子适合形成另一种非苜蓿叶式结构，其中tRNA分子的3 '-一半形成替代发夹，并且tRNA分子被细菌核糖核酸酶P（RNase P）的催化RNA内部切割。到目前为止，tRNA的超加工反应仅在果蝇tRNA中报道过。这一次，我们将超加工反应应用于一种人类tRNA，人类酪氨酸tRNA，我们发现这种tRNA也被E。coli RNase P RNA。这种tRNA是第一个高度加工的非果蝇tRNA的例子。结果表明，超加工反应可以是一个有用的工具，以检测不稳定的tRNA分子从任何物种。
Transfer RNA is an essential molecule for biological system, and each tRNA molecule commonly has a cloverleaf structure. Previously, we experimentally showed that some Drosophila tRNA (tRNA(Ala), tRNA(His), and tRNA(i)(Met)) molecules fit to form another, non-cloverleaf, structure in which the 3'-half of the tRNA molecules forms an alternative hairpin, and that the tRNA molecules are internally cleaved by the catalytic RNA of bacterial ribonuclease P (RNase P). Until now, the hyperprocessing reaction of tRNA has only been reported with Drosophila tRNAs. This time, we applied the hyperprocessing reaction to one of human tRNAs, human tyrosine tRNA, and we showed that this tRNA was also hyperprocessed by E. coli RNase P RNA. This tRNA is the first example for hyperprocessed non-Drosophila tRNAs. The results suggest that the hyperprocessing reaction can be a useful tool to detect destablized tRNA molecules from any species.