Ribonucleic acid-deoxyribonucleic acid hybridization in aqueous solutions and in solutions containing formamide.
Ribonucleic acid-deoxyribonucleic acid hybridization in aqueous solutions and in solutions containing formamide.
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水溶液和含有甲酰胺的溶液中的核糖核酸-脱氧核糖核酸杂交。
DOI：
10.1042/bj1250481
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
D. Gillespie
中科院分区：
文献类型：
--
作者：
S. Gillespie;D. Gillespie
在66℃的6xSSC(0.15M-氯化钠-0.015M-柠檬酸钠)中与35℃下的甲酰胺-6xSSC(1：1，v/v)杂交进行了比较，如预期的那样，RNA杂交电位在前者体系中是不稳定的，在后者中是稳定的。在甲酰胺体系中，过滤器对DNA的保留率很低，但可以改进。对杂交反应的其他几个性质进行了探索，得出结论：甲酰胺体系总体上更优越。
Hybridization in 6xSSC (SSC, 0.15m-sodium chloride-0.015m-sodium citrate) at 66 degrees C was compared with hybridization in formamide-6xSSC (1:1, v/v) at 35 degrees C. As expected, the RNA hybridization potential was labile in the former system and stable in the latter. DNA retention by filters was poor in the formamide system, but could be improved. Several other properties of the hybridization reaction were explored and it was concluded that the formamide system is generally superior.