ANTIGENIC CONSERVATION OF H1N1 SWINE INFLUENZA-VIRUSES
ANTIGENIC CONSERVATION OF H1N1 SWINE INFLUENZA-VIRUSES
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DOI：
10.1099/0022-1317-70-12-3297
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发表时间：
1989-12-01
影响因子：
3.8
通讯作者：
HINSHAW, VS
中科院分区：
文献类型：
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作者：
SHEERAR, MG;EASTERDAY, BC;HINSHAW, VS
H1N1亚型的流感病毒在美国的猪中持续传播至少50年。为了检查这些猪病毒的抗原变异水平，制备了一组60种针对最近猪分离株的血凝素（HA）的单克隆抗体（MAbs）。用这些单克隆抗体选择的中和逃逸突变体的评价定义了HA上的四个抗原位点，其中两个重叠。用这些单克隆抗体对威斯康星州一个流行区24年来分离的猪病毒进行了ELISA和血凝抑制试验，结果表明这些单克隆抗体所确定的抗原位点在这些病毒中高度保守。在比较来自猪、火鸡、鸭和人的最新H1N1病毒时，根据HI反应性检测抗原位点的变化。然而，这些病毒的ELISA结果清楚地表明，抗原位点仍然存在于几乎所有的猪源H1N1病毒;因此，这些病毒在HI试验中与单克隆抗体的反应性改变并不反映单克隆抗体定义的抗原位点的变化。在这些病毒中检测到的变异很可能是通过免疫选择以外的机制发生的。
Influenza viruses of the H1N1 subtypes have been continually circulating in pigs in the U.S.A. for at least 50 years. To examine the level of antigenic variation in these swine viruses, a panel of 60 monoclonal antibodies (MAbs) to the haemagglutinin (HA) of recent swine isolates was prepared. Evaluation of neutralization escape mutants selected with these MAbs defined four antigenic sites on the HA, two of which overlap. Swine viruses isolated over 24 years in an enzootic area in Wisconsin were examined by ELISA and haemagglutination inhibition (HI) with these MAbs and the results indicated that the antigenic sites defined by these MAbs were highly conserved in these viruses. In comparing recent H1N1 viruses from pigs, turkeys, ducks and humans, changes in the antigenic sites were detected on the basis of HI reactivity. However, results of ELISA with these viruses clearly showed that the antigenic sites were still present on almost all H1N1 viruses of swine origin; thus, altered reactivity of these viruses in HI tests with MAbs was not a reflection of changes in the antigenic sites defined by the MAbs. It seems likely that the variation detected in these viruses occurs by a mechanism other than immune selection.