3D Printed Microfluidic Devices for Microchip Electrophoresis of Preterm Birth Biomarkers
3D Printed Microfluidic Devices for Microchip Electrophoresis of Preterm Birth Biomarkers
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DOI：
10.1021/acs.analchem.9b01395
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发表时间：
2019-06-04
影响因子：
7.4
通讯作者：
Woolley, Adam T.
中科院分区：
文献类型：
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作者：
Beauchamp, Michael J.;Nielsen, Anna V.;Woolley, Adam T.
这项工作首次展示了通过立体平版3D打印创建截面尺寸类似于50微米的微芯片电泳仪，并将其应用于与早产风险相关的具有医学意义的生物标志物的分析。我们测得器件电流与高达800V(620V/cm)的外加电压呈线性关系。我们以荧光标记的氨基酸为模型系统优化了器件和分离条件，并将我们的3D打印微流控器件的性能与其他微芯片电泳分析常用的器件材料的性能进行了比较。我们首次在3D打印设备中展示了具有合适分离特性的三个PTB生物标志物，包括多肽和蛋白质。还确定了3D打印微流控设备中微芯片电泳法的检测下限，以确定PTB生物标志物在高皮摩尔到低纳摩尔范围内的检测范围。
This work demonstrates for the first time the creation of microchip electrophoresis devices with similar to 50 mu m cross-sectional dimensions by stereolithographic 3D printing and their application in the analysis of medically significant biomarkers related to risk for preterm birth (PTB). We determined that device current was linear with applied potential up to 800 V (620 V/cm). We optimized device and separation conditions using fluorescently labeled amino acids as a model system and compared the performance in our 3D printed microfluidic devices to that in other device materials commonly used for microchip electrophoresis analysis. We demonstrated for the first time microchip electrophoresis in a 3D printed device of three PTB biomarkers, including peptides and a protein, with suitable separation characteristics. Limits of detection for microchip electrophoresis in 3D printed microfluidic devices were also determined for PTB biomarkers to be in the high picomolar to low nanomolar range.