INSITU OXYGEN-CONSUMPTION RATES OF CELLS IN V-79 MULTICELLULAR SPHEROIDS DURING GROWTH
INSITU OXYGEN-CONSUMPTION RATES OF CELLS IN V-79 MULTICELLULAR SPHEROIDS DURING GROWTH
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DOI：
10.1002/jcp.1041180111
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发表时间：
1984-01-01
影响因子：
5.6
通讯作者：
SUTHERLAND, RM
中科院分区：
文献类型：
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作者：
FREYER, JP;TUSTANOFF, E;SUTHERLAND, RM
多细胞球体中V-79细胞的耗氧率作为球体直径的函数被测量。对于直径小于200µm的球体，原位消耗等于指数生长的细胞的消耗。直径在200到400微米之间的球体细胞的耗氧率下降到最初的1/4，然后随着球体的进一步生长保持不变。比较从球体结构解离前后球体来源的细胞的耗氧率，发现球体微环境仅占氧气消耗率变化的20%。细胞接触、细胞包装和细胞体积不是关键参数。平台期细胞的耗氧率比指数期细胞低5倍，推测椭圆形静止细胞群是椭圆形细胞耗氧量内在变化的主要原因。V-79球体细胞氧耗的调节必须涉及其他机制，以解释在该系统中观察到的剩余减少。
The rate of O2 consumption by V-79 cells in multicellular spheroids was measured as a function of the spheroid diameter. In situ consumption was equal to that of exponentially growing cells for spheroids less than 200 .mu.m in diameter. The rate of O2 consumption decreased for cells in spheroids between 200 and 400 .mu.m diameter to a value 1/4 the initial, then remained constant with further spheroid growth. Comparison of consumption rates for spheroid-derived cells before and after dissociation from the spheroid structure indicated that the spheroid microenvironment accounted for only 20% of the change in O2 consumption rate. Cell-cell contact, cell packing and cell volume were not critical parameters. Plateau-phase cells had a 5-fold lower rate of O2 consumption than exponential cells, and it is postulated that the spheroid quiescent cell population accounts for a large part of the intrinsic alteration in O2 consumption of cells in spheroids. Some other mechanism must be involved in the regulation of cellular O2 consumption in V-79 spheroids to account for the remainder of the reduction observed in this system.