A SINGLE DOMAIN OF YEAST POLY(A)-BINDING PROTEIN IS NECESSARY AND SUFFICIENT FOR RNA-BINDING AND CELL VIABILITY
A SINGLE DOMAIN OF YEAST POLY(A)-BINDING PROTEIN IS NECESSARY AND SUFFICIENT FOR RNA-BINDING AND CELL VIABILITY
复制标题
DOI：
10.1128/mcb.7.9.3268
复制
发表时间：
1987-09-01
影响因子：
5.3
通讯作者：
KORNBERG, RD
中科院分区：
文献类型：
--
作者：
SACHS, AB;DAVIS, RW;KORNBERG, RD
聚(A)结合蛋白（PAB）基因是酿酒酵母细胞生长所必需的基因。含有一半重复n端结构域的66个氨基酸的多肽可以在体内取代整个蛋白质。无论是在真核生物rna结合蛋白中保守的八肽序列，还是PAB的c端结构域，都不需要在体内发挥作用。在体外聚(A)结合的高亲和力位点数量上，单个n端结构域与整个蛋白几乎相同（一个位点的关联常数约为2倍）。107 M-1)和结合位点的大小（12个A残基）。多个n端结构域提供了聚(a)链之间PAB转移的机制。
The poly(A)-binding protein (PAB) gene of Saccharomyces cerevisiae is essential for cell growth. A 66-amino acid polypeptide containing half of a repeated N-terminal domain can replace the entire protein in vivo. Neither an octapeptide sequence conserved among eucaryotic RNA-binding proteins nor the C-terminal domain of PAB is required for function in vivo. A single N-terminal domain is nearly identical to the entire protein in the number of high-affinity sites for poly(A) binding in vitro (one site with an association constant of approximately 2 .times. 107 M-1) and in the size of the binding site (12 A residues). Multiple N-terminal domains afford a mechanism of PAB transfer between poly(A) strands.