Ecdysone-responsive transcriptional regulation determines the temporal expression of cuticular protein genes in wing discs of Bombyx mori
Ecdysone-responsive transcriptional regulation determines the temporal expression of cuticular protein genes in wing discs of Bombyx mori
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DOI：
10.1016/j.gene.2012.09.126
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发表时间：
2013-01-10
期刊：
影响因子：
3.5
通讯作者：
Kawasaki, Hideki
中科院分区：
文献类型：
--
作者：
Ali, Md Saheb;Iwanaga, Masashi;Kawasaki, Hideki
本研究旨在阐明家蚕预蛹期翅盘表达的角质层蛋白基因的调控机制。 BHR3、BHR4、E74A 和 β FTZ-F1 在蛹前阶段的翅盘中相继表达。 BHR3 显示出与其他蜕皮激素反应性转录因子 (ERTF) 不同的蜕皮激素反应性，并且是通过添加蜕皮激素诱导的，但在治疗后 (蜕皮激素脉冲) 去除蜕皮激素后表现出降低。 BHR4和E74A由蜕皮激素添加和蜕皮激素脉冲诱导。 β FTZ-F1 不是由添加蜕皮激素诱导的，而是由蜕皮激素脉冲诱导的。因此，ERTF表现出不同的激素反应性，导致不同的表达时间。我们选择了与每个转录因子表现出相同表达阶段的角质蛋白基因，并检查了它们的蜕皮激素反应性。 BmorCPH5、BmorCPR34、BmorCPR23 和 BmorCPR99 的发育表达和蜕皮激素反应性分别与 BHR3、BHR4、E74A 和 β FTZ-F1 相似。瞬时报告基因测定的结果强烈表明 ERTF 对每个角质层蛋白启动子的调节。这些 ERTF 调节不同的角质层蛋白基因，并决定它们的表达时间，并可能决定昆虫角质层的性质。 (c) 2012 Elsevier B.V. 保留所有权利。
The present study was conducted to clarify the regulatory mechanism of cuticular protein genes of Bombyx mori expressed in wing discs in the prepupal stage. BHR3, BHR4, E74A, and beta FTZ-F1 were successively expressed in wing discs at the pre-pupal stage. BHR3 showed different ecdysone responsiveness from other ecdysone-responsive transcription factors (ERTFs) and was induced by ecdysone addition but showed decrease by ecdysone removal after treatment (ecdysone pulse). BHR4 and E74A were induced by the ecdysone addition and by the ecdysone pulse. beta FTZ-F1 was not induced by the ecdysone addition but was induced by the ecdysone pulse. Thus, ERTFs showed different hormone responsiveness, resulting in the different expression timing. We selected cuticular protein genes that showed the same expression stage with each transcription factor and examined their ecdysone responsiveness. The developmental expression and ecdysone responsiveness of BmorCPH5, BmorCPR34, BmorCPR23, and BmorCPR99 resembled those of BHR3, BHR4, E74A, and beta FTZ-F1, respectively. The results of the transient reporter assay strongly suggested the regulation of each cuticular protein promoter by ERTF. These ERTFs regulated different cuticular protein genes and determined their expression timing and probably the nature of the cuticle layer of insects. (c) 2012 Elsevier B.V. All rights reserved.