Investigating the role of BN-domains of FlhF involved in flagellar synthesis in Campylobacter jejuni
Investigating the role of BN-domains of FlhF involved in flagellar synthesis in Campylobacter jejuni
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研究 FlhF BN 结构域在空肠弯曲杆菌鞭毛合成中的作用
DOI：
10.1016/j.micres.2021.126944
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发表时间：
2022-03-01
影响因子：
6.7
通讯作者：
Huang, Jinlin
中科院分区：
文献类型：
--
作者：
Li, Xiaofei;Chai, Qinwen;Huang, Jinlin
FlhF蛋白是空肠弯曲菌完整鞭毛组装的关键。它是一个推定的具有B-、N-和G-结构域的GT酶。然而，Band N结构域在鞭毛生物合成中的作用在C.空肠。这项研究表明，这两个带N-结构域是必需的鞭毛合成，与B和/或N-结构域的情况下，显示截短的变体FlhF的TEM。Band N-结构域（T13 A、K159 A、G231 A）的点突变也诱导鞭毛异常。此外，显着的缺陷，GTdR活性和极性靶向的FlhF被触发的带N-结构域的点突变。flhF（T13 A）、flhF（K159 A）和flhF（G231 A）菌株中鞭毛基因的表达和转录也被显著破坏。本研究初步探讨了Band N结构域对鞭毛合成的影响。我们推测Band N-结构域可能通过影响鞭毛基因表达或GTbR活性直接或间接导致鞭毛异常，这有助于我们更好地理解FlhF在鞭毛合成中的功能。
FlhF protein is critical for intact flagellar assembly in Campylobacter jejuni. It is a putative GTPase with B-, Nand G-domains. However, the role of the Band N-domains in flagella biosynthesis remains unclear in C. jejuni. This study demonstrated that both the Band N-domains are essential for flagellar synthesis, with the absence of B and/or N-domains showing truncated variants of FlhF by TEM. Point mutations in the Band N-domains (T13A, K159A, G231A) also induced flagella abnormalities. Furthermore, significant defects in GTPase activity and polar targeting of FlhF were triggered by point mutations of Band N-domains. Flagella gene expression and transcription were also significantly disrupted in flhF(T13A), flhF(K159A) and flhF(G231A) strains. This study initially explored the effects of Band N-domains on flagella synthesis. We speculated that Band N-domains may directly or indirectly cause flagella abnormalities by affecting flagellar gene expression or GTPase activity, which helps us better understand the function of FlhF in flagella synthesis.