Enhancement of Firefly Bioluminescence Using Liposomes Containing Cationic Components
Enhancement of Firefly Bioluminescence Using Liposomes Containing Cationic Components
复制标题
使用含有阳离子成分的脂质体增强萤火虫生物发光
DOI：
10.2116/analsci.14.1115
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发表时间：
1998
影响因子：
1.6
通讯作者：
Hiroto Watanabe
中科院分区：
文献类型：
--
作者：
T. Kamidate;T. Fujita;H. Tani;Hiroto Watanabe
利用挤压技术(VET)形成的囊泡，研究了脂质体中阳离子组分对萤火虫荧光素-荧光素酶与三磷酸腺苷(ATP)反应生物发光强度的影响。以硬脂酰三甲基氯化铵(STAC)、硬脂胺(SA)和丁卡因(TC)为阳离子组分。含有STAC和SA的VET增强了最大蓝光强度。相反，在含有TC的VET存在的情况下，观察到最大BL强度的降低。在含有STAC的VET存在下，ATP的灵敏度比在含有SA的VET存在下和在水中单独存在时分别提高2倍和10倍。不同阳离子组分的电致发光增强的差异可以用含有阳离子组分的VET膜表面电位的不同来解释。
The effect of cationic components in liposome on the bioluminescence (BL) intensity from the firefly luciferin-luciferase reaction with ATP was investigated by use of vesicles formed by the extrusion technique (VET). Stearyltrimethyl-ammonium chloride (STAC), stearylamine (SA) and tetracaine (TC) were used as cationic components. The VET containing STAC and SA enhanced the maximum BL intensity. In contrast, a lowering of the maximum BL intensity was observed in the presence of the VET containing TC. The sensitivity for ATP in the presence of the VET containing STAC was improved by a factor of 2 and 10 times compared to that in the presence of the VET containing SA and that in water alone, respectively. The differences in the BL enhancement between cationic components could be explained in terms of different membrane surface potentials of the VET containing these cationc components.