MORE EFFICIENT POSITIVE SELECTION OF THYMOCYTES IN MICE LACKING TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE
MORE EFFICIENT POSITIVE SELECTION OF THYMOCYTES IN MICE LACKING TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE
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DOI：
10.1093/intimm/6.11.1681
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发表时间：
1994-11-01
影响因子：
4.4
通讯作者：
MATHIS, D
中科院分区：
文献类型：
--
作者：
GILFILLAN, S;WALTZINGER, C;MATHIS, D
具有末端脱氧核苷酸转移酶（TdT）基因剧烈突变的小鼠最近被改造。这些小鼠的Igs和tcr基本上缺乏n区多样性。在这里，我们报告了TdT(o)小鼠含有CD3（hi）单阳性（SP）胸腺细胞数量的增加，因为更多的胸腺细胞从未成熟的双阳性过渡到成熟的SP阶段。这表明，在种系中编码的tcr库可能丰富了能够与MHC分子相互作用的特异性，并且这种亲和力的一些丧失是为tdt产生的多样性付出的代价。
Mice with a drastic mutation in the terminal deoxynucleotidyl transferase (TdT) gene have recently been engineered. Igs and TCRs in these mice are essentially devoid of N-region diversity. Here we report that TdT(o) mice contain elevated numbers of CD3(hi) single-positive (SP) thymocytes because more thymocytes make the transition from the immature double-positive to the mature SP stage. This suggests that the repertoire of TCRs encoded in the germline may be enriched for specificities capable of interacting with MHC molecules and that the loss of some of this affinity is the price paid for TdT-generated diversity.