Blue light postpones senescence of carnation flowers through regulation of ethylene and abscisic acid pathway-related genes
Blue light postpones senescence of carnation flowers through regulation of ethylene and abscisic acid pathway-related genes
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DOI：
10.1016/j.plaphy.2020.03.018
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发表时间：
2020-06-01
影响因子：
6.5
通讯作者：
Serek, Margrethe
中科院分区：
文献类型：
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作者：
Aalifar, Mostafa;Aliniaeifard, Sasan;Serek, Margrethe
内源信号对外源因子的响应决定了花的衰老。植物激素尤其是脱落酸（阿坝）和乙烯之间的相互作用是植物衰老的主要决定因素。本研究以香石竹切花为材料，研究了内源信号阿坝和乙烯相关基因的复杂表达模式。暴露在不同的光谱下。在暴露于三种光谱[白色、蓝色和红色]的康乃馨花瓣中评估乙烯生物合成（DcACS和DcACO）和信号传导（DcETR和DcEin 2）基因以及参与阿坝生物合成（DcZEP 1和DcNCED 1）、运输（DcABCG 25和DcABCG 40）和catalysis（DcCYP 707 A1）的基因的表达。蓝光处理的花的相对膜透性最低，瓶插寿命最长。红光和白色光分别在瓶插后第6天和第10天显著加速了花的衰老，并增加了DcACS和DcACO的表达，而蓝光则抑制了乙烯生物合成基因的表达。参与阿坝的生产和运输和乙烯的信号转导的基因的表达升高，在瓶插寿命的花，无论暴露于不同的光谱。可见，蓝光通过下调乙烯生物合成基因和上调阿坝生物合成基因，延缓花瓣衰老，是延长香石竹瓶插寿命的有效环境因子。
Endogenous signals in response to exogenous factors determine the senescence of flowers. Interactions among phytohormones especially abscisic acid (ABA) and ethylene are the major determinant of the senescence. In the present study, complex expression patterns of the genes related to ABA and ethylene as endogenous signals were investigated on cut carnations (Dianthus caryophyllus L.) that were exposed to different light spectra. Expression of ethylene biosynthetic (DcACS and DcACO), and signaling (DcETR and DcEin2) genes and also genes involved in ABA biosynthesis (DcZEP1 and DcNCED1), transport (DcABCG25 and DcABCG40) and catabolism (DcCYP707A1) were evaluated in petals of carnations exposed to three light spectra [white, blue and red]. Lowest relative membrane permeability (RMP) was detected in flowers that exposed to Blue light (BLFs), as a consequence, the longest vase life was found in BLFs. The Red and White lights markedly accelerated flower senescence and increased expression of DcACS and DcACO on day 6 and 10 of vase life assessment respectively; while Blue light inhibited the expression of ethylene biosynthetic genes. Expression of the genes involved in the production and transport of ABA and in signal transduction of ethylene was elevated during vase life of flowers irrespective of exposure to different light spectra. In conclusion, Blue light can be an effective environmental factor to extend the vase life of carnation flowers by delaying the petal senescence through down-regulation of ethylene biosynthetic genes and up-regulation of ABA biosynthetic genes.