Evidence for recent intergeneric transfer of a new tetracycline resistance gene, tet(W), isolated from Butyrivibrio fibrisolvens, and the occurrence of tet(O) in ruminal bacteria
Evidence for recent intergeneric transfer of a new tetracycline resistance gene, tet(W), isolated from Butyrivibrio fibrisolvens, and the occurrence of tet(O) in ruminal bacteria
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DOI：
10.1046/j.1462-2920.1999.00004.x
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发表时间：
1999-02-01
影响因子：
5.1
通讯作者：
Flint, HJ
中科院分区：
文献类型：
--
作者：
Barbosa, TM;Scott, KP;Flint, HJ
我们以前曾报道过四环素耐药性的高频率转移之间的瘤胃厌氧细菌溶纤维丁酸弧菌菌株。假定供体菌株携带两个Tc-R基因，其中一个转移到新的染色体元件上。这里显示，在B中的非传递性基因内的编码序列。fibrisolvens 1.230与肺炎链球菌泰特（O）基因的那些相同。这为兼性厌氧细菌和瘤胃专性厌氧细菌之间的遗传交换提供了第一个证据。相反，可传递的Tc-R基因的产物与TetO和TetM蛋白的氨基酸序列同源性仅为68%，代表了一类新的核糖体保护四环素抗性决定簇，命名为泰特W。泰特（W）编码区的DNA G + C含量（53%）高于其它B。fibrisolvens基因或其他核糖体保护型泰特基因，表明最近从高G + C含量基因组获得。序列几乎相同的泰特（W）基因也显示存在于B的Tc-R菌株中。这为最近瘤胃细菌之间抗性基因的属间转移提供了令人信服的证据。泰特（W）不限于瘤胃细菌，因为它也存在于猪M.多酸的
We have previously reported high-frequency transfer of tetracycline resistance between strains of the rumen anaerobic bacterium Butyrivibrio fibrisolvens. Donor strains were postulated to carry two Tc-R genes, one of which is transferred on a novel chromosomal element. It is shown here that coding sequences within the non-transmissible gene in B. fibrisolvens 1.230 are identical to those of the Streptococcus pneumoniae tet(O) gene. This provides the first evidence for genetic exchange between facultatively anaerobic bacteria and rumen obligate anaerobes, In contrast, the product of the transmissible Tc-R gene shares only 68% amino acid sequence identity with the TetO and TetM proteins and represents a new class of ribosome protection tetracycline resistance determinant, designated Tet W. The tet(W) coding region shows a higher DNA G + C content (53%) than other B. fibrisolvens genes or other ribosome protection-type tet genes, suggesting recent acquisition from a high G + C content genome. Tet(W) genes with almost identical sequences are also shown to be present in Tc-R strains of B. fibrisolvens from Australian sheep and in Tc-R strains of two other genera of rumen obligate anaerobes, Selenomonas ruminantium and Mitsuokella multiacidus, This provides compelling evidence for recent intergeneric transfer of resistance genes between ruminal bacteria. Tet(W) is not restricted to ruminal bacteria, as it was also present in a porcine strain of M. multiacidus.