Osteoclast and osteoblast response to strontium-doped struvite coatings on titanium for improved bone integration
Osteoclast and osteoblast response to strontium-doped struvite coatings on titanium for improved bone integration
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DOI：
10.1515/bmt-2019-0265
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发表时间：
2020-05
期刊：
影响因子：
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通讯作者：
C. Moseke;Katharina Wimmer;M. Meininger;J. Zerweck;C. Wolf-Brandstetter;U. Gbureck;A. Ewald
中科院分区：
文献类型：
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作者：
C. Moseke;Katharina Wimmer;M. Meininger;J. Zerweck;C. Wolf-Brandstetter;U. Gbureck;A. Ewald
摘要 为了开发改善骨向内生长的种植体，通过电化学辅助沉积的方法在钛基体上涂覆均匀致密的鸟粪石（MgNH4PO4·6H2O）层。将不同浓度的硝酸锶添加到涂层电解液中，以制备 Sr 负载量为 10.6 至 115 μg/cm2 的 Sr 掺杂鸟粪石涂层。预计并观察到植入物周围的破骨细胞活性受到抑制。体外分析了涂层的细胞相容性以及不同浓度的Sr离子对破骨细胞形成的影响。从形态学、生物化学以及基因表达水平上阐明了破骨细胞的分化。结果表明，中等浓度的 Sr2+ 对破骨细胞形成有抑制作用，而与纯鸟粪石表面相比，成骨细胞的生长没有受到负面影响。总之，电化学沉积的掺锶鸟粪石涂层是改善骨植入物向内生长的一种有前途的方法。
Abstract To develop implants with improved bone ingrowth, titanium substrates were coated with homogeneous and dense struvite (MgNH4PO4·6H2O) layers by means of electrochemically assisted deposition. Strontium nitrate was added to the coating electrolyte in various concentrations, in order to fabricate Sr-doped struvite coatings with Sr loading ranging from 10.6 to 115 μg/cm2. It was expected and observed that osteoclast activity surrounding the implant was inhibited. The cytocompatibility of the coatings and the effect of Sr-ions in different concentrations on osteoclast formation were analyzed in vitro. Osteoclast differentiation was elucidated on morphological, biochemical as well as on gene expression level. It could be shown that moderate concentrations of Sr2+ had an inhibitory effect on osteoclast formation, while the growth of osteoblastic cells was not negatively influenced compared to pure struvite surfaces. In summary, the electrochemically deposited Sr-doped struvite coatings are a promising approach to improve bone implant ingrowth.