Efficiency of Single Phage Suspensions and Phage Cocktail in the Inactivation of Escherichia coli and Salmonella Typhimurium: An In Vitro Preliminary Study
Efficiency of Single Phage Suspensions and Phage Cocktail in the Inactivation of Escherichia coli and Salmonella Typhimurium: An In Vitro Preliminary Study
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DOI：
10.3390/microorganisms7040094
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发表时间：
2019-04-01
期刊：
影响因子：
4.5
通讯作者：
Almeida, Adelaide
中科院分区：
文献类型：
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作者：
Costa, Pedro;Pereira, Carla;Almeida, Adelaide
肠杆菌科大肠杆菌和肠沙门氏菌血清型鼠伤寒杆菌菌株是造成医院和社区环境中中度和严重感染的主要病原体，部分原因是它们经常对抗生素产生耐药性。由于肠杆菌科感染的治疗是经验性的，使用相同的抗生素治疗大肠杆菌和沙门氏菌感染，同样的概念可以应用于噬菌体。将不同的噬菌体混合在鸡尾酒中，经常用于避免噬菌体耐药突变体的发展，也允许治疗多种病原体，扩大噬菌体的作用谱。因此，本研究的目的是评估两种噬菌体（大肠杆菌上产生的ELY-1和鼠伤寒沙门氏菌上产生的phSE-5）的混合物控制大肠杆菌和鼠伤寒沙门氏菌的效率。噬菌体ly -1和phase -5对大肠杆菌（最大减少量分别为4.5和3.8 log CFU/mL）、鼠伤寒沙门氏菌（最大减少量分别为2.2和2.6 log CFU/mL）以及两种细菌的混合物（最大减少量分别为2.2和2.0 log CFU/mL）均有效。鸡尾酒ELY-1/ phase -5对鼠伤寒沙门氏菌和这两种细菌的混合物比单个噬菌体悬浮液更有效（两者最大减少3.2 log CFU/mL），对大肠杆菌的效果与其特异性噬菌体ELY-1一样（最大减少4.5 log CFU/mL）。然而，使用噬菌体鸡尾酒和单噬菌体悬浮液并没有阻止噬菌体抗性突变体的发生。总的来说，结果表明，以鸡尾酒形式应用噬菌体显示了它们的潜在用途，即在病原体鉴定之前，为其用于控制大肠杆菌或鼠伤寒沙门氏菌铺平道路。
Enterobacteriaceae Escherichia coli and Salmonella enterica serovar Typhimurium strains are among the main pathogens responsible for moderate and serious infections at hospital and community environments, in part because they frequently present resistance to antibiotics. As the treatment of Enterobacteriaceae infections is empiric, using the same antibiotics to treat E. coli and Salmonella infections, the same concept can be applied with phages. The use of different phages combined in cocktails, frequently used to circumvent the development of phage-resistant mutants, also allows for the treatment of multiple pathogens, broadening the phages' action spectrum. As such, the aim of this study was to evaluate the efficiency of a cocktail of two phages (ELY-1, produced on E. coli and phSE-5, produced on S. Typhimurium) to control E. coli and S. Typhimurium. Phages ELY-1 and phSE-5 were effective against E. coli (maximum reductions of 4.5 and 3.8 log CFU/mL, respectively), S. Typhimurium (maximum reductions of 2.2 and 2.6 log CFU/mL, respectively), and the mixture of both bacteria (maximum reductions of 2.2 and 2.0 log CFU/mL, respectively). The cocktail ELY-1/phSE-5 was more effective against S. Typhimurium and the mixture of both bacteria (maximum reduction of 3.2 log CFU/mL for both) than the single phage suspensions and as effective against E. coli as its specific phage ELY-1 (maximum reductions of 4.5 log CFU/mL). The use of both the phage cocktails, as well as the single-phage suspensions, however, did not prevent the occurrence of phage-resistant mutants. Overall, the results indicate that the application of the phages in the form of a cocktail show their potential to be used presumptively, that is, prior to the identification of the pathogens, paving its use to control E. coli or S. Typhimurium.