The microbiome of the oral mucosa in irritable bowel syndrome
The microbiome of the oral mucosa in irritable bowel syndrome
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DOI：
10.1080/19490976.2016.1162363
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发表时间：
2016-01-01
期刊：
影响因子：
12.2
通讯作者：
Henderson, Wendy A.
中科院分区：
文献类型：
--
作者：
Fourie, Nicolaas H.;Wang, Dan;Henderson, Wendy A.
肠易激综合症（IBS）是一种人们知之甚少的疾病，其特征是持续症状，包括内脏疼痛。研究证明了炎症性肠病中口腔微生物组的差异，表明口腔微生物组在非口腔疾病研究中的潜力。在这项探索性研究中，我们研究了 IBS 参与者和健康对照者的口腔微生物组是否存在差异，以及口腔微生物组是否与症状严重程度相关。使用 PhyloChip 微阵列对 38 名参与者的口腔颊粘膜微生物组进行了表征。通过口服胃肠道测试溶液来评估内脏疼痛的严重程度。参与者自我报告了他们诱发的内脏疼痛。 IBS 参与者的疼痛严重程度最高 (P = 0.0002)，特别是 IBS 超重参与者 (P = 0.02)，并且与 60 个 OTU、4 个属、5 个科和 4 个目细菌的丰度密切相关 (r(2) > 0.4，P < 0.001)。 IBS 超重参与者的拟杆菌门 (P = 0.007) 和芽孢杆菌属 (P = 0.008) 丰富度下降。 β-多样性分析发现 IBS 超重组存在显着差异（P < 0.05）。我们的口腔微生物结果与所描述的粪便和结肠微生物组-IBS 和体重关联一致。患有肠易激综合症和超重，而不是肠易激综合症亚型，是描述内脏疼痛严重程度和微生物群变化的最重要因素。疼痛的严重程度与许多分类群的丰度密切相关，这表明口腔微生物组在诊断和患者表型分析中的潜力。口腔微生物组有潜力作为 IBS 微生物信息的来源。
Irritable bowel syndrome (IBS) is a poorly understood disorder characterized by persistent symptoms, including visceral pain. Studies have demonstrated oral microbiome differences in inflammatory bowel diseases suggesting the potential of the oral microbiome in the study of non-oral conditions. In this exploratory study we examine whether differences exist in the oral microbiome of IBS participants and healthy controls, and whether the oral microbiome relates to symptom severity. The oral buccal mucosal microbiome of 38 participants was characterized using PhyloChip microarrays. The severity of visceral pain was assessed by orally administering a gastrointestinal test solution. Participants self-reported their induced visceral pain. Pain severity was highest in IBS participants (P = 0.0002), particularly IBS-overweight participants (P = 0.02), and was robustly correlated to the abundance of 60 OTUs, 4 genera, 5 families and 4 orders of bacteria (r(2) > 0.4, P < 0.001). IBS-overweight participants showed decreased richness in the phylum Bacteroidetes (P = 0.007) and the genus Bacillus (P = 0.008). Analysis of beta-diversity found significant separation of the IBS-overweight group (P < 0.05). Our oral microbial results are concordant with described fecal and colonic microbiome-IBS and -weight associations. Having IBS and being overweight, rather than IBS-subtypes, was the most important factor in describing the severity of visceral pain and variation in the microbiome. Pain severity was strongly correlated to the abundance of many taxa, suggesting the potential of the oral microbiome in diagnosis and patient phenotyping. The oral microbiome has potential as a source of microbial information in IBS.