RETROTRANSPOSITION OF A PLANT SINE INTO THE WX LOCUS DURING EVOLUTION OF RICE
RETROTRANSPOSITION OF A PLANT SINE INTO THE WX LOCUS DURING EVOLUTION OF RICE
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DOI：
10.1007/bf00166160
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发表时间：
1994-02-01
影响因子：
3.9
通讯作者：
SANO, Y
中科院分区：
文献类型：
--
作者：
HIRANO, HY;MOCHIZUKI, K;SANO, Y
在水稻（Olyza sariva）wx基因座中发现了一种新的植物反转录子p-SINE 1。它具有一些类似于哺乳动物西内斯的结构特征，例如Alu或B1家族的成员。为了估计p-SINE 1整合到一个单一位点的时间发生在水稻进化过程中，我们研究了两个成员的p-SINE 1在几个物种的稻属聚合酶链反应（PCR）。我们发现wx（+）基因第九内含子中的一个p-SINE 1成员（p-SINE 1-r2）仅存在于两个近缘种O. sativa和O. Rifipogon的AA基因组，并不存在于其他物种携带的AA基因组在稻属。该结果表明，p-SINE 1-r2在O. sativa和O. rufipogon与AA基因组的其他物种有分歧。与p-SINE 1-r2相反，位于wx（+）基因非翻译区的另一个成员（p-SINE 1-r1）不仅存在于所有AA基因组物种中，而且存在于具有不同基因组的物种（CCDD）中。该结果表明，p-SINE 1-r1在基因组趋异之前整合到该位置。因此，这表明p-SINE 1的每个成员在水稻进化过程中的不同时间在特定位点上被逆转录。
A new type of plant retroposon, p-SINE1, has been found in the wx locus of rice (Olyza sariva). It has some structural characteristics similar to those of mammalian SINEs, such as members of the Alu or B1 family. In order to estimate the time at which the integration of p-SINE1 into a single locus occurred during rice evolution, we examined the distribution of two members of p-SINE1 in several species of the Oryza genus by the polymerase chain reaction (PCR). We found that one member of p-SINE1 (p-SINE1-r2) in the ninth intron of the wx(+) gene was present only in two closely related species, O. sativa and O. rifipogon, and was not present in the other species carrying the AA genome within the Oryza genus. This result indicates that p-SINE1-r2 was integrated into the wx locus after O. sativa and O. rufipogon had diverged from other species with the AA genome. In contrast to p-SINE1-r2, another member (p-SINE1-r1) located in the untranslated 5'-region of the wx(+) gene was present not only in all species with the AA genome but also in species with a different genome (CCDD). This result suggests that p-SINE1-r1 was integrated into that position prior to the genomic divergence. Thus, it appears that each member of p-SINE1 was retroposed at a specific site at a different time during rice evolution.