Fluorescence correlation spectroscopy: inception, biophysical experimentations, and prospectus.
Fluorescence correlation spectroscopy: inception, biophysical experimentations, and prospectus.
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DOI：
10.1364/ao.40.003969
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发表时间：
2001-08
期刊：
影响因子：
1.9
通讯作者：
Watt W. Webb
中科院分区：
文献类型：
--
作者：
Watt W. Webb
荧光相关光谱法通过测量几个分子的荧光的动态光学波动来检查稀分子溶液的化学和物理动力学，甚至一次平均不到一个分子，在通常小于1毫微微升（<10（-18）m（3））的开放焦点体积中。它应用了与准弹性光散射相同的统计热力学原理。在分子荧光在动态范围10（-7）-10（2）s内变化的情况下，通常不能通过准弹性光散射检测到的分子相互作用、构象变化、化学反应和生物物理动力学可以通过荧光相关光谱法进行分析。
Fluorescence correlation spectroscopy examines the chemical and the photophysical dynamics of dilute molecular solutions by measurement of the dynamic optical fluctuations of the fluorescence of a few molecules, even averaging less than one molecule at a time, in open focal volumes that are usually less than a femtoliter (<10(-18) m(3)). It applies the same principles of statistical thermodynamics as does quasi-elastic light scattering. Molecular interactions, conformational changes, chemical reactions, and photophysical dynamics that are not ordinarily detectable by quasi-elastic light scattering can be analyzed by fluorescence correlation spectroscopy in cases in which molecular fluorescence changes in the dynamic range 10(-7)-10(2) s.