Congruence between parental reproductive investment and success determined by DNA-based pedigree reconstruction in conifer seed orchards
Congruence between parental reproductive investment and success determined by DNA-based pedigree reconstruction in conifer seed orchards
复制标题
DOI：
10.1139/x10-190
复制
发表时间：
2011-02-01
影响因子：
2.2
通讯作者：
El-Kassaby, Yousry A.
中科院分区：
文献类型：
--
作者：
Funda, Tomas;Liewlaksaneeyanawin, Cherdsak;El-Kassaby, Yousry A.
种子园的亲本繁殖成功率通常是通过调查种子和花粉的生产（生殖投资）。虽然这种方法的可靠性受到质疑，但其速度和简便性使其成为提供种子果园作物遗传质量和数量近似估计的首选方法。在这项研究中，生殖投资数据从各种女性和男性表型评估方法从三个种子园（黑松（Pinus contorta Dougl。ex.大声了变种宽叶黄（Latifolia Engelm.），花旗松（Pseudotsuga menziesii（Mirb.）Franco）和西部落叶松（Larix oceanentalis Nutt.）与基于DNA指纹和亲子关系分析的繁殖成功估计进行了比较。评价了生殖投资作为生殖成功的替代指标在估算种子作物产量、遗传价值和多样性（以有效亲本数衡量）方面的应用。回归分析表明，表型评估方法反映了真实的父母繁殖成功，估计种子和花粉锥体积生产似乎是最合适的指标女性和男性繁殖成功，分别。虽然最佳组合的女性-男性的调查方法产生膨胀的有效数量的父母估计，遗传价值的估计是类似的那些基于DNA，突出了他们的稳健性生育变异评估。
Seed orchards' parental reproductive success is often assessed through surveys of seed-and pollen-cone production (reproductive investment). Although the reliability of this approach has been questioned, its speed and ease made it the method of choice for providing approximate estimates for the genetic quality and quantity of seed orchard crops. In this study, reproductive investment data from various female and male phenotypic assessment methods from three seed orchards (lodgepole pine (Pinus contorta Dougl. ex. Loud. var. latifolia Engelm.), Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco), and western larch (Larix occidentalis Nutt.)) were compared with reproductive success estimates based on DNA fingerprinting and parentage analyses. The use of reproductive investment as a proxy to reproductive success on estimating seed crop's volume genetic worth and diversity (measured by effective number of parents) was evaluated. Regression analyses indicated that the phenotypic assessment methods reflect the true parental reproductive success and that estimates of seed-and pollen-cone volume production appear to be the most appropriate indicators of female and male reproductive success, respectively. Although the best combination of female-male survey methods produced inflated effective number of parents estimates, the genetic worth estimates were similar to those based on DNA, highlighting their robustness to fertility variation assessment.