Immunohistochemical detection of proliferating cell nuclear antigen and p53 expression in carcinoma of the extrahepatic bile duct.
Immunohistochemical detection of proliferating cell nuclear antigen and p53 expression in carcinoma of the extrahepatic bile duct.
复制标题
免疫组织化学检测肝外胆管癌增殖细胞核抗原及p53表达。
DOI：
10.1159/000227727
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发表时间：
1997
期刊：
影响因子：
3.5
通讯作者：
Kazuyoshi Saito
中科院分区：
文献类型：
--
作者：
Takayuki Suto;T. Sugai;Shin‐ichi Nakamura;N. Uesugi;Ryouko Sasaki;S. Kanno;Kazuyoshi Saito
我们用免疫组化方法测定p53和增殖细胞核抗原（PCNA）的浓度，以评估其作为恶性肿瘤的预测指标的有用性，并研究PCNA和p53在肝外胆管癌（EHBD）中的关系。用PC 10和DO 7单克隆抗体分别对46例石蜡包埋标本进行PCNA和p53免疫组化染色。PCNA标记指数（LI）与肿瘤的分期、浸润深度密切相关（p < 0.05）。单因素分析发现PCNA LI低者（LI < 47%）的累积生存率显著高于PCNA LI高者（LI ≥ 47%）（p < 0.05），但多因素分析PCNA LI不是独立的预后因素。我们在37%的EHBD癌症中检测到p53。我们还发现p53阳性与PCNA标记细胞的百分比或存活率无关。结果表明，PCNA免疫反应性可能是一个有用的预测恶性肿瘤患者的EHBD。
We used an immunohistochemical method to determine the concentrations of p53 and proliferating cell nuclear antigen (PCNA) to evaluate their usefulness as a predictor of malignancy and examined the relationship between PCNA and p53 in carcinomas of the extrahepatic bile duct (EHBD). Paraffin-embedded specimens from 46 patients were immunostained for PCNA and p53 using PC10 and DO7 monoclonal antibodies, respectively. The PCNA labeling index (LI) was closely associated with the stages of the tumor and depth of invasion (p < 0.05). The cumulative survival rate of patients with a low PCNA LI (LI < 47%) was found to be significantly better than that of patients with a high PCNA LI (LI > or = 47%) in all cases and patients with advanced cancer by univariate analysis (p < 0.05), but PCNA LI was not an independent prognostic factor in multivariate analysis. We detected p53 in 37% of the EHBD cancers. We also found that p53 positivity was not related to the percentage of PCNA-labelled cells or survival. The results suggest that PCNA immunoreactivity may be a useful predictor of malignancy in patients with EHBD carcinomas.