The capacity of silage inoculant bacteria to bind aflatoxin B1 in vitro and in artificially contaminated corn silage
The capacity of silage inoculant bacteria to bind aflatoxin B1 in vitro and in artificially contaminated corn silage
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DOI：
10.3168/jds.2016-12370
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发表时间：
2017-09-01
影响因子：
3.5
通讯作者：
Adesogan, A. T.
中科院分区：
文献类型：
--
作者：
Ma, Z. X.;Amaro, F. X.;Adesogan, A. T.
目的是检查青贮饲料细菌的黄曲霉毒素 B-1 (AFB1) 结合能力以及影响反应的因素。实验 1 和 2 检查了细菌菌株和种群对 10 种细菌的 AFB(1) 结合能力的影响。当以 10(6) cfu/mL 应用于体外培养基时，仅植物乳杆菌 PT5B 结合 AFB(1)，且结合能力较低 (4%)。当以 10(9) cfu/mL 施用时，所有 10 种细菌均结合 AFB(1)，但植物乳杆菌 R2014 (Lp) 和 EQ12、布氏乳杆菌 R1102 (Lb) 以及乳酸片球菌 R2142 和 EQ01 (Pa) 的结合能力最大（23.9 至 33%）。实验 3 检查了 Lp、Lb 和 Pa 在不同 pH 下的活和非活（HCl 处理）形式的 AFB(1) 结合能力。无法存活的 Lb 和 Lp（而非 Pa）增加了 AFB(1) 结合。 AFB(1) 的结合在 pH 2.5 时最强，在 pH 8 时最低。由于实验 3 中结合 AFB(1) 的非活性 Lb 和 Lp 不是有效的青贮饲料接种剂，实验 4 检查了良性处理与严重处理的效果（85 与 100 摄氏度；pH 2.5 与 100 摄氏度）。
The objectives were to examine the aflatoxin B-1 (AFB1)-binding capacity of silage bacteria and factors affecting the responses. Experiments 1 and 2 examined the effects of bacterial strain and population on the AFB(1)-binding capacity of 10 bacteria. When applied at 10(6) cfu/mL to an in vitro medium, only Lactobacillus plantarum PT5B bound the AFB(1) and the binding capacity was low (4%). When applied at 10(9) cfu/mL, all 10 bacteria bound AFB(1), but L. plantarum R2014 (Lp) and EQ12, Lactobacillus buchneri R1102 (Lb), and Pediococcus acidilactici R2142 and EQ01 (Pa) had the greatest capacity (23.9 to 33%). Experiment 3 examined the AFB(1)-binding capacity of viable and nonviable (HCl-treated) forms of Lp, Lb, and Pa at different pH. Nonviable Lb and Lp, but not Pa, increased AFB(1) binding. Binding of AFB(1) was greatest at pH 2.5 and least at pH 8. As the nonviable Lb and Lp that bound AFB(1) in experiment 3 would not be effective silage inoculants, experiment 4 examined effects of benign versus severe treatments (85 vs. 100 degrees C; pH 2.5 vs.