Proteomic analysis of Lactococcus lactis, a lactic acid bacterium
Proteomic analysis of Lactococcus lactis, a lactic acid bacterium
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DOI：
10.1002/pmic.200390047
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发表时间：
2003-03-01
期刊：
影响因子：
3.4
通讯作者：
Mistou, MY
中科院分区：
文献类型：
--
作者：
Guillot, A;Gitton, C;Mistou, MY
乳酸乳球菌是革兰氏阳性菌，属于乳酸菌群，其中有几个属在食品生产中起着至关重要的作用。在M17培养基中培养乳酸乳杆菌IL1403细胞质蛋白，采用两种pH梯度（pH 4-7、4.5-5.5）对其进行双向凝胶电泳。肽质量指纹图谱鉴定了230多个位点，占预测酸性蛋白质组的25%。目前的研究使得在蛋白质组水平上描述与重要生理过程和技术特性相关的大量细胞途径（糖酵解、发酵、核苷酸代谢、蛋白质水解、脂肪酸和肽聚糖合成）成为可能。这也表明乳酸乳杆菌的发酵代谢与糖酵解酶的高表达有关。34个蛋白质与没有指定功能的开放阅读框相匹配。两株乳酸菌在蛋白质组水平上的比较显示出重要的蛋白质多态性。葡萄糖和乳糖生长的细胞的蛋白质组的比较揭示了碳源的性质和嘧啶核苷酸代谢之间意想不到的联系。
Lactococcus lactis is a Gram-positive bacteria, which belongs to the group of lactic acid bacteria among which several genera play an essential role in the manufacture of food products. Cytosolic proteins of L. lactis IL1403 cultivated in M17 broth have been resolved by two-dimensional gel electrophoresis using two pH gradients (pH 4-7, 4.5-5.5). More than 230 spots were identified by peptide mass fingerprints, corresponding to 25% of the predicted acid proteome. The present study made it possible to describe at the proteome level a significant number of cellular pathways (glycolysis, fermentation, nucleotide metabolism, proteolysis, fatty acid and peptidoglycan synthesis) related to important physiological processes and technological properties. It also indicated that the fermentative metabolism, which characterizes L. lactis is associated with a high expression of glycolytic enzymes. Thirty-four proteins were matched to open reading frames for which there is no assigned function. The comparison at the proteome level of two strains of L. lactis showed an important protein polymorphism. The comparison of the proteomes of glucose- and lactose-grown cells revealed an unexpected link between the nature of the carbon source and the metabolism of pyrimidine nucleotides.