Modification of the Campylobacter jejuni flagellin glycan by the product of the Cj1295 homopolymeric-tract-containing gene
Modification of the Campylobacter jejuni flagellin glycan by the product of the Cj1295 homopolymeric-tract-containing gene
复制标题
DOI：
10.1099/mic.0.038091-0
复制
发表时间：
2010-07-01
期刊：
影响因子：
2.8
通讯作者：
Linton, Dennis
中科院分区：
文献类型：
--
作者：
Hitchen, Paul;Brzostek, Joanna;Linton, Dennis
空肠弯曲菌鞭毛蛋白是由九碳糖伪氨基酸的结构类似物O-糖基化而成。空肠弯曲菌81-176株最常见的修饰是5，7-二-N-乙酰化衍生物(Pse5Ac7Ac)和乙酰氨基取代形式(Pse5Am7Ac)。检测到的其他结构包括O-乙酰化和N-乙酰谷氨酰胺取代的衍生物(分别为Pse5Am7Ac8OAc和Pse5Am7Ac8GInNAc)。最近，在空肠弯曲菌NCTC 11168菌株中检测到一种二氧甲基甘油酰基修饰的伪氨基酸衍生物。Pse5Ac7Ac生物合成所需的基因产物已经被鉴定，但那些参与产生其他结构的基因还没有。我们已经证明了NCTC 11168鞭毛蛋白在十二烷基硫酸钠-PAGE凝胶中的迁移率可以自发变化，我们研究了鞭毛蛋白糖基化位点上的单核苷酸重复序列或含有同源多聚体的基因在这一过程中的作用。其中一个名为CJ1295的基因被证明与鞭毛糖蛋白的结构变化有关。质谱学分析表明，CJ1295基因是与二-O-甲基甘油酰化的伪氨基酸糖基化所必需的。
The Campylobacter jejuni flagellin protein is O-glycosylated with structural analogues of the nine-carbon sugar pseudaminic acid. The most common modifications in the C. jejuni 81-176 strain are the 5,7-di-N-acetylated derivative (Pse5Ac7Ac) and an acetamidino-substituted version (Pse5Am7Ac). Other structures detected include O-acetylated and N-acetylglutamine-substituted derivatives (Pse5Am7Ac8OAc and Pse5Am7Ac8GInNAc, respectively). Recently, a derivative of pseudaminic acid modified with a di-O-methylglyceroyl group was detected in C. jejuni NCTC 11168 strain. The gene products required for Pse5Ac7Ac biosynthesis have been characterized, but those genes involved in generating other structures have not. We have demonstrated that the mobility of the NCTC 11168 flagellin protein in SDS-PAGE gels can vary spontaneously and we investigated the role of single nucleotide repeats or homopolymeric-tract-containing genes from the flagellin glycosylation locus in this process. One such gene, Cj1295, was shown to be responsible for structural changes in the flagellin glycoprotein. Mass spectrometry demonstrated that the Cj1295 gene is required for glycosylation with the di-O-methylglyceroyl-modified version of pseudaminic acid.