Immunoreactive endorphins, lipotropins and corticotropins in a human nonpituitary tumor: evidence for a common precursor.
Immunoreactive endorphins, lipotropins and corticotropins in a human nonpituitary tumor: evidence for a common precursor.
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人类非垂体肿瘤中的免疫反应性内啡肽、促脂蛋白和促肾上腺皮质激素：共同前体的证据。
DOI：
10.1210/jcem-46-5-849
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
W. Nicholson
中科院分区：
文献类型：
--
作者：
D. Orth;R. Guillemin;N. Ling;W. Nicholson
用凝胶排阻层析法测定了引起异位ACTH综合征的人胰岛细胞癌提取物中的免疫反应性（RIA）、ACTH、LPH、α内啡肽（α End）和β End。除了“大”、“中”和“小”ACTH、β促脂素（β LPH）和γ LPH外，β-LPH组分还含有RIA-α末端和-β末端。主要的RIA-β End组分似乎是h β LPH，其羧基末端为β End，但也发现了与合成的p β End共洗脱的显著浓度的组分。在两个峰中发现了少量的RIA-α End，一个可能代表1-76 h β LPH，羧基末端为α End，另一个可能代表α End本身。在空隙体积分数中发现RIA-ACTH、-LPH和-β End。因此，产生ACTH和LPH的人类非垂体肿瘤也产生内啡肽，所有这些可能都来自于一个共同的前体。
The immunoreactive (RIA), ACTH, LPH, alpha endorphin (alpha End) and beta End in an extract of a human pancreatic islet cell carcinoma causing the ectopic ACTH syndrome were assayed after gel exclusion chromatography. In addition to "big", "intermediate" and "little" ACTHs, beta lipotropin (beta LPH) and gamma LPH, the eluate fractions contained RIA-alpha End and -beta End. The major RIA-beta End component appeared to be h beta LPH, which has beta End as its carboxy-terminus, but significant concentrations of a component that coeluted with synthetic p beta End were also found. Small amounts of RIA-alpha End were found in two peaks, one that may have represented 1-76 h beta LPH, with alpha End as its carboxy-terminus, and one probably representing alpha End itself. RIA-ACTH, -LPH and -beta End were found in the void volume fractions. Thus, a human nonpituitary tumor that produced ACTH and LPHs also produced endorphins, all perhaps deriving from a common precursor.