Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection
Identification of the amino acid-AZT-phosphoramidase by affinity T7 phage display selection
复制标题
DOI：
10.1016/j.bmcl.2009.09.067
复制
发表时间：
2009-11-15
影响因子：
2.7
通讯作者：
Wagner, Carston R.
中科院分区：
文献类型：
--
作者：
Cheng, Jilin;Zhou, Xin;Wagner, Carston R.
制备了CEM细胞的T7噬菌体展示文库，并用于筛选AZT单磷酸盐的磷酰胺前药激活酶。尽管与基于核糖核苷酸的磷酰胺相比，hHint 1的效率较低，但hHint 1被鉴定为可能的细胞内原核苷酸激活酶。(C)爱思唯尔有限公司出版的2009年。
A CEM cell cDNA T7 phage display library was prepared and used to screen for activating enzymes of phosphoramidate prodrugs of AZT monophosphate. Although, inefficient compared to ribonucleotide based phosphoramidates, hHint 1 was identified as the likely intracellular pronucleotide activating enzyme. (C) 2009 Published by Elsevier Ltd.