INTERACTION OF BILE-ACIDS, PHOSPHOLIPIDS, CHOLESTEROL AND TRIGLYCERIDE WITH DIETARY-FIBERS IN THE SMALL-INTESTINE OF RATS
INTERACTION OF BILE-ACIDS, PHOSPHOLIPIDS, CHOLESTEROL AND TRIGLYCERIDE WITH DIETARY-FIBERS IN THE SMALL-INTESTINE OF RATS
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DOI：
10.1093/jn/119.8.1100
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发表时间：
1989-08-01
影响因子：
4.2
通讯作者：
SCHNEEMAN, BO
中科院分区：
文献类型：
--
作者：
EBIHARA, K;SCHNEEMAN, BO
据报道，某些膳食纤维通过结合胆汁酸并减少其通过肠肝循环的再循环来降低血浆胆固醇。此外，某些纤维可能会延缓脂肪的消化和吸收。研究了胆汁酸与瓜尔胶（GG）、魔芋甘露聚糖（KM）和壳聚糖（CH）的相互作用。在200分钟的时间内，给大鼠喂食含有5%纤维素（CE）、GG、KM或CH以及含有14c标记的三油酯和3h标记的胆固醇的试验餐。饲喂CE组作为对照组，因为CE在体内不结合胆汁酸和磷脂。在提供试验餐两小时后，杀死大鼠，切除胃、小肠和大肠。所有四组都吃了相同量的测试餐，大约1.9克。采用超离心分离法分离小肠内容物的水相，测定肠道总内容物和水相中胆汁酸和磷脂的含量（μ mol）。与CE组相比，GG组和KM组水相胆汁酸与肠道总内容物的比值显著升高，CH组显著降低，说明胆汁酸被这两种纤维源结合或捕获。只有CH似乎与磷脂结合，与CE组相比，水相中的比例减少。在从胃中排出的14C和3H中，GG和KM组留在小肠中的比例高于CE组，这表明由于喂养GG或KM，小肠对胆固醇和甘油三酯的吸收都被推迟了。
Certain dietary fibers have been reported to lower plasma cholesterol by binding bile acids and reducing their recycling through the enterohepatic circulation. In addition, certain fibers may delay the digestion and absorption of fat. In the present study, the interaction of bile acids with guar gum (GG), konjac mannan (KM) and chitosan (CH) was determined. Rats were fed during a 200min period a test meal containing either 5% cellulose (CE), GG, KM or CH and also containing 14C-labeled triolein and 3H-labeled cholesterol. The group fed CE served as control, since CE does not bind bile acids or phospholipids in vivo. Two hours after presentation of the test meal, rats were killed and the stomach and small and large intestine removed. All four groups ate the same amount of the test meal, about 1.9 g. The aqueous phase of the small intestinal contents was separated by ultracentrifugation, and the amount (.mu.mol) of bile acids and phospholipids in the total intestinal contents and in the aqueous phase was estimated. The ratio of bile acids in the aqueous phase to that in total intestinal contents was significantly higher in the GG and KM groups and significantly lower in the CH group than that in the CE group, demonstrating that the bile acids are bound or trapped by each of these fiber sources. Only CH appeared to bind phospholipids, reducing the proportion in the aqueous phase compared to that in the CE group. Of the 14C and 3H emptied from the stomach, a greater percentage remained in the small intestine of the GG and KM groups than in that of the CE groups, indicating that absorption of both cholesterol and triglyceride from the small intestine was delayed due to feeding GG or KM.