A lipase isolated from the silkworm Bombyx mori shows antiviral activity against nucleopolyhedrovirus
A lipase isolated from the silkworm Bombyx mori shows antiviral activity against nucleopolyhedrovirus
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DOI：
10.1128/jvi.77.19.10725-10729.2003
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发表时间：
2003-10-01
影响因子：
5.4
通讯作者：
Yamakawa, M
中科院分区：
文献类型：
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作者：
Ponnuvel, KM;Nakazawa, H;Yamakawa, M
从家蚕B消化液中分离纯化了一种抗家蚕核型多角体病毒（BmNPV）的蛋白。桑果幼虫。推导的氨基酸序列的同源性搜索的蛋白质cDNA显示与果蝇脂肪酶的同源性为56%，与人类脂肪酶的同源性为21%。由于在体外证实了该蛋白质的脂肪酶活性，因此将该蛋白质命名为Bmlipase-1。北方印迹分析表明，Bmlipase-1基因在中肠中表达，但在其他组织中不表达，也不被BmNPV感染激活。此外，Bmlipase-1基因在蜕皮期和游走期不表达，表明该基因受卵巢调节。我们的研究结果表明，昆虫消化酶具有潜在的生理屏障，对BmNPV在病毒感染的初始位置。
A protein showing strong antiviral activity against Bombyx mori nucleopolyhedrovirus (BmNPV) was purified from the digestive juice of B. mori larvae. A homology search of the deduced amino acid sequence of the protein cDNA revealed 56% homology with Drosophila melanogaster lipase and 21% homology with human lipase. As lipase activity of the protein was confirmed in vitro, this protein was designated Bmlipase-1. Northern blot analysis showed that the Bmlipase-1 gene is expressed in the midgut but not in other tissues, nor is it activated by BmNPV infection. In addition, the Bmlipase-1 gene was shown not to be expressed in the molting and wandering stages, indicating that the gene is hormonally regulated. Our results suggest that an insect digestive enzyme has potential as a physiological barrier against BmNPV at the initial site of viral infection.