Analysis of Learning and Memory Ability in an Alzheimer's Disease Mouse Model using the Morris Water Maze
Analysis of Learning and Memory Ability in an Alzheimer's Disease Mouse Model using the Morris Water Maze
复制标题
使用莫里斯水迷宫分析阿尔茨海默病小鼠模型的学习和记忆能力
DOI：
10.3791/60055
复制
发表时间：
2019-10-01
影响因子：
1.2
通讯作者：
Li, Zhigang
中科院分区：
文献类型：
--
作者：
Tian, Huiling;Ding, Ning;Li, Zhigang
莫里斯水迷宫（MWM）实验迫使实验动物游泳，并学习寻找隐藏在水中的平台。它被广泛用于科学研究，以评估动物的学习和记忆。由于MWM测试的广泛使用，可视化实验方案对研究人员至关重要。本文利用最新的研究成果介绍了MWM试验的方案。阿尔茨海默病（AD）的特征在于记忆和认知功能的进行性丧失。用于AD的替代和补充治疗是手动针灸（MA）。采用MWM实验评价AD模型小鼠的学习记忆能力。采用MWM的可见平台试验、隐藏平台试验、探索试验和逆转试验评价大鼠的空间学习记忆能力。可见平台实验中，各组小鼠的游泳速度和逃避潜伏期无显著差异。在隐藏平台和逆转实验中，AD组表现出较长的逃避潜伏期。MA处理后逃避潜伏期明显缩短。MA治疗后，探测试验中低平台交叉数和SW象限中的时间比例增加（p < 0.05或p < 0.01）。MWM实验结果表明，MA能有效改善AD模型小鼠的空间学习记忆能力。严格的实验操作保证了结果的可靠性。
A Morris water maze (MWM) experiment forces experimental animals to swim and learn to find a platform hidden in the water. It is widely used in scientific research to assess the learning and memory of animals. Due to the extensive use of the MWM test, visual experimental protocols are essential for researchers. This manuscript uses the latest studies to introduce the protocol of the MWM test. Alzheimer' Disease (AD) is characterized by a progressive loss of memory and cognitive function. An alternative and complementary treatment used for AD is Manual Acupuncture (MA). To assess the learning and memory ability of AD model mice, the MWM test was conducted. The visible platform trial, hidden platform trial, probe trial, and reversal trial of MWM were used to evaluate spatial learning and memory ability. In the visible platform trial, the swimming speed and escape latency of mice in different groups was not significantly different. In the hidden platform and reversal trials, the AD group showed a long escape latency. The escape latency decreased significantly after the MA treatment. Low platform crossover number and the proportion of time in the SW quadrant in the probe trial increased after the MA treatment (p < 0.05 or p < 0.01). The results of the MWM tests suggest that MA can effectively improve the spatial learning and memory abilities of AD model mice. Rigorous experimental operations provided assurance of the reliability of the results.