How schistosomes alter the human serum proteome
How schistosomes alter the human serum proteome
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DOI：
10.1016/j.molbiopara.2016.12.007
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发表时间：
2017-07-01
影响因子：
1.5
通讯作者：
Skelly, Patrick J.
中科院分区：
文献类型：
--
作者：
Da'dara, Akram A.;Siddons, Giles;Skelly, Patrick J.
血吸虫是血管内的寄生虫，可引起使人衰弱的血吸虫病。为了更好地了解这些长寿的寄生虫是如何破坏宿主的免疫和止血能力的，我们在这里研究了成年曼氏血吸虫对人类血清蛋白质组的影响。正常人血清（150 μ l）在37℃下孵育1小时，有或没有成虫（50对）。然后去除寄生虫，对血清样品进行标记，并通过2d -差分凝胶电泳（2D-DIGE）对其蛋白进行分析。两个样本之间有几个不同之处。通过胰蛋白酶消化和质谱分析，提取并鉴定了20个蛋白点。引人注目的是，其中大多数（11/20）与补体系统有关，包括补体成分C3， C4，因子B，补体因子H相关蛋白1和聚集蛋白。免疫印迹分析证实了线虫对血清中C3、C4和因子B的影响。这些数据表明，血吸虫与补体接触，但可以迅速降解选定的补体蛋白，这可能有助于解释蠕虫对补体介导的攻击的难耐性。其他被血吸虫侵染的血清蛋白有α - 2巨球蛋白、α - 1抗凝乳胰蛋白酶、肌动蛋白细胞质2、血清淀粉样蛋白A-4、蛋白DDX26B、血红蛋白亚基B和血清白蛋白。虽然这些蛋白与血吸虫相互作用的分子性质尚不清楚，但其中一些蛋白可能在止血、免疫逃避和宿主对血清应激的反应中发挥作用。(C) 2017 Elsevier B.V.版权所有
Schistosomes are intravascular parasitic worms that cause the debilitating disease schistosomiasis. To better understand how these long-lived parasites may subvert host immune and hemostatic capabilities, we examine here the impact of adult Schistosoma mansoni worms on the human serum proteome. Normal human serum (150 mu l) was incubated at 37 degrees C for one hour either in the presence or absence of adult worms (similar to 50 pairs). Thereafter parasites were removed, serum samples were labeled and their proteins resolved for comparative analysis by 2D-Differential in-Gel Electrophoresis (2D-DIGE). Several differences were noted between the two samples. Twenty protein spots were recovered and identified following trypsin digestion and mass spectroscopy. Strikingly, most of these (11/20) are associated with the complement system and include complement components C3, C4, factor B, complement factor H related protein 1 and clusterin. Western blot analysis confirms the impact of the worms on C3, C4 and factor B in serum. The data suggest that schistosomes engage complement but can rapidly degrade selected complement proteins which may help explain the worm's refractoriness towards complement mediated attack. Other serum proteins identified as being impinged upon by schistosomes are alpha 2 macroglobulin, alpha 1 anti-chymotrypsin, actin cytoplasmic 2, serum amyloid A-4, protein DDX26B, hemoglobin subunit B and serum albumin. While the molecular nature of the interaction between these proteins and schistosomes is not known, possible roles for some of them in hemostasis, immune evasion and in the host response to serum stress are suggested. (C) 2017 Elsevier B.V. All rights reserved.