RNA helicase activity of the plant virus movement proteins encoded by the first gene of the triple gene block
RNA helicase activity of the plant virus movement proteins encoded by the first gene of the triple gene block
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DOI：
10.1006/viro.2001.1328
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发表时间：
2002-05-10
期刊：
影响因子：
3.7
通讯作者：
Morozov, SY
中科院分区：
文献类型：
--
作者：
Kalinina, NO;Rakitina, DV;Morozov, SY
一些植物病毒的细胞间和长距离运输需要三重基因块(TGB)编码的三个运动蛋白的协调作用。TGB蛋白中最大的TGBp1是DNA/RNA解旋酶超家族I的成员，具有一套保守的解旋酶序列基序，是病毒运动所必需的。在大肠杆菌中产生的两种霍尔德病毒和马铃薯X病毒的His标记的重组TGBp1在部分双链RNA上具有解离活性，但在DNA底物上没有解离活性。这些蛋白的解旋酶活性依赖于镁离子和三磷酸腺苷。分离的PSLV TGBp1的C末端一半保留了所有的解旋酶基序，也能够解开RNA双链。(C)2002年埃尔塞维尔科学公司(美国)。
Cell-to-cell and long-distance transport of some plant viruses requires coordinated action of three movement proteins encoded by triple gene block (TGB). The largest of TGB proteins, TGBp1, is a member of the superfamily I of DNA/RNA helicases and possesses a set of conserved helicase sequence motifs necessary for virus movement. A recombinant His-tagged form of TGBp1 of two hordeiviruses and potato virus X, a potexvirus, produced in Escherichia coli had unwinding activity on a partially duplexed RNA, but not DNA substrate. The helicase activity of these proteins was dependent on Mg2+ and ATP. The isolated C-terminal half of the PSLV TGBp1 retaining all helicase motifs was also able to unwind RNA duplex. (C) 2002 Elsevier Science (USA).