Molecular characterization and nutritional regulation of specificity protein 1 (Sp1) in grass carp (Ctenopharyngodon idella)
Molecular characterization and nutritional regulation of specificity protein 1 (Sp1) in grass carp (Ctenopharyngodon idella)
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草鱼（Ctenopharyngodon idella）特异性蛋白1（Sp1）的分子特征和营养调节
DOI：
10.1111/anu.12630
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发表时间：
2018-06-01
影响因子：
3.5
通讯作者：
Liu, Z.
中科院分区：
文献类型：
--
作者：
Qu, F.;Tang, J.;Liu, Z.
特异性蛋白1（Specificity protein 1，Sp1）是真核细胞中普遍存在的一种核因子，在调节肠内二肽/三肽的吸收过程中起着重要作用。然而，Sp1在硬骨鱼中的功能仍然知之甚少。本研究从草鱼（Ctenopharyngodon idella）中克隆了一个鱼类Sp1基因（命名为CiSp 1）。推导的CiSp 1蛋白包含Sp1家族蛋白的典型结构特征，包括富含谷氨酰胺（Q-丰富）的区域和三个典型的Cys 2 His 2锌指。实时荧光定量PCR分析表明，CiSp 1在草鱼的所有测试组织和胚胎发育阶段的普遍表达。此外，在喂食鱼粉和270 g/kg膳食蛋白后，肠道中CiSp 1的表达显著增加。此外，在体外和体内谷氨酰胺二肽处理显示出类似的刺激效应的转录因子CiSp 1和CiCDX 2及其潜在的靶基因CiPepT 1在草鱼肠道的表达。最后，我们的结果表明，CiSp 1/CiCDX 2/CiPepT 1在肠道中的mRNA表达显着增加后，丁酸钠在体外和体内处理。总之，这些结果首次表明，草鱼具有功能性Sp1，其表达受饲料蛋白和饲料添加剂的调节。
Specificity protein 1 (Sp1) is a ubiquitous nuclear factor in eukaryotic cells and plays a crucial role in the regulation of di-/tripeptide absorption in the intestine. However, the function of Sp1 in bony fish remains poorly understood. In this study, a fish Sp1 gene (designated CiSp1) was identified from Ctenopharyngodon idella. The deduced CiSp1 protein contains structural features typical of Sp1 family proteins, including a glutamine-rich (Q-rich) region and three typical Cys2His2 zinc fingers. Quantitative real-time PCR analysis revealed ubiquitous expression of CiSp1 in all tested tissues and embryonic developmental stages of grass carp. In addition, CiSp1 expression in the intestine was significantly increased after feeding with fish meal and 270 g/kg dietary protein. Moreover, in vitro and in vivo glutamine dipeptide treatments showed similar stimulatory effects on the expression of the transcription factors CiSp1 and CiCDX2 and their potential target gene CiPepT1 in the intestines of grass carp. Finally, our results demonstrated that the mRNA expression of CiSp1/CiCDX2/CiPepT1 in the intestine was significantly increased after treatment with sodium butyrate in vitro and in vivo. Altogether, these results demonstrate for the first time that grass carp possesses a functional Sp1 and that its expression is regulated by dietary protein and feed additives.