Polymers for the rapid and effective activation and aggregation of platelets
Polymers for the rapid and effective activation and aggregation of platelets
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DOI：
10.1016/j.biomaterials.2011.06.001
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发表时间：
2011-10-01
期刊：
影响因子：
14
通讯作者：
Bradley, Mark
中科院分区：
文献类型：
--
作者：
Hansen, Anne;McMillan, Loraine;Bradley, Mark
血小板负责堵塞血管损伤部位，激活后血小板会扩散并交联，防止进一步失血。对于诸如创伤后快速止血等应用来说，需要控制激活过程。聚合物是许多生物领域的首选材料，其物理特性可以控制形态并易于功能化和生产。在此，聚合物微阵列用于筛选复杂的人体液体（富含血小板的血浆），以鉴定可用于调节血小板活化的聚丙烯酸酯。通过 CD61P 结合确定了几种快速激活血小板的聚合物，随后通过扫描电子显微镜分析进行确认。这种方法可以直接比较天然激动剂胶原蛋白和合成聚合物之间的血小板活化状态以及结合血小板的数量。生理流下的进一步研究表明，静态微阵列实验为潜在的医学应用提供了可行的候选者，同时与聚合物的特定蛋白质结合被确定为可能的作用模式。该方法证明了聚合物微阵列能够识别特定生物激活事件的新聚合物，并且在这种情况下，允许识别在静态和流动研究中比天然标准胶原蛋白在高级激活状态下允许更高水平的血小板结合的材料。 Crown 版权所有 (C) 2011 由 Elsevier Ltd 出版。保留所有权利。
Platelets are responsible for plugging sites of vascular injury, where upon activation they spread out and become cross-linked, preventing further blood loss. It is desirable to control the activation process on demand for applications such as the rapid staunching of blood flow following trauma. Polymers are the material of choice in many biological areas, with physical properties that allow control of morphology as well as ease of functionalisation and production. Herein, polymer microarrays were used to screen a complex human fluid (platelet rich plasma) to identify polyacrylates that could be used to modulate platelet activation. Several polymers were identified which rapidly activated platelets as determined by CD61P binding and subsequent confirmation by scanning electron microcopy analysis. This approach enabled a direct comparison between the natural agonist collagen and synthetic polymers with respect to the activation status of the platelets as well as the number of bound platelets. Further investigations under physiological flow demonstrated that the static microarray experiments gave viable candidates for potential medical applications while specific protein binding to the polymers was identified as a possible mode of action. The approach demonstrates the ability of polymer microarrays to identify new polymers for specific biological activation events and in this case allowed the identification of materials that allowed higher levels of platelets to bind in advanced activation states than the natural standard collagen in static and flow studies. Crown Copyright (C) 2011 Published by Elsevier Ltd. All rights reserved.