B-cell activating factor receptor expression is associated with germinal center B-cell maintenance
B-cell activating factor receptor expression is associated with germinal center B-cell maintenance
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DOI：
10.3892/etm.2019.7172
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发表时间：
2019-03-01
影响因子：
2.7
通讯作者：
Azucena Palafox-Sanchez, Claudia
中科院分区：
文献类型：
--
作者：
Josue Carrillo-Ballesteros, Francisco;Oregon-Romero, Edith;Azucena Palafox-Sanchez, Claudia
B 细胞激活因子 (BAFF) 是一种主要细胞因子，通过与其受体结合来调节 B 细胞存活、成熟和分化：BAFF 受体 (BAFF-R)、跨膜激活剂和亲环蛋白配体相互作用蛋白 (TACI) 和 B 细胞成熟抗原 (BCMA)。这些受体已被证明参与三级淋巴结构的形成；然而，它们在生发中心（GC）中的作用仍然难以捉摸。本研究的目的是确定 BAFF 及其受体在次级淋巴组织中的表达谱。因慢性扁桃体炎而切除的扁桃体被用作淋巴组织。为了确认根据其典型结构识别的 GC 的存在，采用了 CD21 抗体染色。通过免疫组织化学评估 BAFF、BAFF-R、TACI 和 BCMA 的表达。 BAFF 在毛囊的所有区域均高表达，但在地幔区 (MZ) 检测到最高的 BAFF 表达。与其他区域相比，在 MZ 的淋巴细胞中观察到 BAFF-R 高表达（接近 80%；P
B-cell activating factor (BAFF) is a major cytokine that regulates B-cell survival, maturation and differentiation through its binding with its receptors: BAFF receptor (BAFF-R), transmembrane activator and cyclophilin ligand interactor (TACI) and B-cell maturation antigen (BCMA). These receptors have been demonstrated to be involved in tertiary lymphoid structure formation; however, their role in germinal centers (GCs) has remained elusive. The aim of the present study was to determine the expression profiles of BAFF and its receptors in secondary lymphoid tissues. Tonsils resected due to chronic tonsillitis were used as lymphoid tissues. To confirm the presence of GCs identified based on their typical structure, CD21 antibody staining was employed. The expression of BAFF, BAFF-R, TACI and BCMA was assessed by immunohistochemistry. BAFF was highly expressed in all regions of the follicle, but the highest BAFF expression was detected in the mantle zone (MZ). A high expression of BAFF-R was observed on lymphocytes in the MZ in comparison with the other regions (similar to 80%; P