DNA fiber autoradiography of tobacco mesophyll protoplasts
DNA fiber autoradiography of tobacco mesophyll protoplasts
复制标题
烟草叶肉原生质体DNA纤维放射自显影
DOI：
10.1007/bf00327711
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发表时间：
1976
期刊：
影响因子：
1.6
通讯作者：
T. Nagata
中科院分区：
文献类型：
--
作者：
T. Nitta;T. Nagata
DNA纤维放射自显影技术成功地应用于烟草叶肉原生质体的分离。孵育37小时后，开始用3 H-胸苷标记DNA。这些细胞，与再生的薄细胞壁，然后有效地破坏在含有十二烷基硫酸钠的裂解液。DNA纤维放射自显影证实，该植物的平均复制子大小约为60 μ，与先前在动物细胞中报道的值非常一致。
DNA fiber autoradiography was successfully applied to protoplasts isolated from tobacco leaf mesophyll. After an incubation period of 37 h, DNA began to be labeled with3H-thymidine. These cells, with regenerated thin cell walls, were then effectively disrupted in a lytic solution containing sodium dodecyl sulfate. DNA fiber autoradiography confirmed that the mean replicon size of this plant is approximately 60 μ in good agreement with the values reported previously in animal cells.