RAPID FOCUS REDUCTION NEUTRALIZATION TEST OF JAPANESE ENCEPHALITIS-VIRUS IN MICROTITER SYSTEM - BRIEF REPORT
RAPID FOCUS REDUCTION NEUTRALIZATION TEST OF JAPANESE ENCEPHALITIS-VIRUS IN MICROTITER SYSTEM - BRIEF REPORT
复制标题
DOI：
10.1007/bf01314119
复制
发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
TAKAGI, M
中科院分区：
文献类型：
--
作者：
OKUNO, Y;FUKUNAGA, T;TAKAGI, M
建立了一种改进的乙脑病毒快速减斑中和试验。96-用微孔组织培养板制备细胞单层，用PAP（过氧化物酶-抗过氧化物酶）染色技术观察感染细胞的病灶。由于该方法具有许多其他方法无法比拟的优点，因此在快速诊断和血清流行病学研究中有着广泛的应用前景。
An improved rapid focus reduction neutralization test for Japanese encephalitis (JE) virus in microtiter system was developed. 96-well tissue culture plates were used for preparation of cell monolayers and PAP (peroxidase-antiperoxidase) staining technique was used for visualization of foci of infected cells. As the test has many advantages over other methods, it might be applied widely for rapid diagnosis and seroepidemiological studies.